Formacao de ácidos orgánicos, acúcares e lipídeos a partir de acetato C em folhas de cafeeiro
The acetate 14C metabolism was studied in detached coffee leaves (Coffea arabica L. cv. 'Mundo Novo'). The leaves were incubated in 10 pCi of acetato-1-C and 10 uCi of acetato-12-25C out of Inminosity Incubation was interrupted adding HCI after 15, 30, 45, 60, 90, 120, 150 and 180 minutes. One insol...
| Main Authors: | , |
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| Format: | Artículo |
| Language: | Portugués |
| Published: |
Instituto Interamericano de Cooperación para la Agricultura (IICA)
2023
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| Online Access: | https://repositorio.catie.ac.cr/handle/11554/12474 |
| Summary: | The acetate 14C metabolism was studied in detached coffee leaves (Coffea arabica L. cv. 'Mundo Novo'). The leaves were incubated in 10 pCi of acetato-1-C and 10 uCi of acetato-12-25C out of Inminosity Incubation was interrupted adding HCI after 15, 30, 45, 60, 90, 120, 150 and 180 minutes. One insoluble fraction and one soluble (80% ethanol) fraction were obtained. Ion exhange cromatography separated she sugar from the organic acids fraction.
Coffee detached leaves used acelafe-14C 10 shymtbesize organic acids, sugar and lipids.
The high activity in organic acid and CO2, indicated that acetate was used via the Krebs cycle. |
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