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Formacao de ácidos orgánicos, acúcares e lipídeos a partir de acetato C em folhas de cafeeiro

Formacao de ácidos orgánicos, acúcares e lipídeos a partir de acetato C em folhas de cafeeiro

The acetate 14C metabolism was studied in detached coffee leaves (Coffea arabica L. cv. 'Mundo Novo'). The leaves were incubated in 10 pCi of acetato-1-C and 10 uCi of acetato-12-25C out of Inminosity Incubation was interrupted adding HCI after 15, 30, 45, 60, 90, 120, 150 and 180 minutes....

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Bibliographic Details
Main Authors: Brasil, O.G., Crocomo, O.J.
Format: Artículo
Language:Portugués
Published: Instituto Interamericano de Cooperación para la Agricultura (IICA) 2023
Subjects:
Coffea||Coffea||Coffea||Coffea
Coffea arabica||Coffea arabica||Coffea arabica||Coffea arabica
Ácidos orgánicos||organic acids||ácido orgânico||acide organique
Hojas||leaves||folha||feuille
Composición||composition||undefined||composition
Azucares||sugars||açúcares||sucres
Lípidos||lipids||lípido||lipide
Metabolismo||metabolism||metabolismo||métabolisme
Acétatos||acetates||acetato||acétate
Sede Central
Online Access:https://repositorio.catie.ac.cr/handle/11554/12474
Description
Summary:The acetate 14C metabolism was studied in detached coffee leaves (Coffea arabica L. cv. 'Mundo Novo'). The leaves were incubated in 10 pCi of acetato-1-C and 10 uCi of acetato-12-25C out of Inminosity Incubation was interrupted adding HCI after 15, 30, 45, 60, 90, 120, 150 and 180 minutes. One insoluble fraction and one soluble (80% ethanol) fraction were obtained. Ion exhange cromatography separated she sugar from the organic acids fraction. Coffee detached leaves used acelafe-14C 10 shymtbesize organic acids, sugar and lipids. The high activity in organic acid and CO2, indicated that acetate was used via the Krebs cycle.

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