Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72%...

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Main Authors:	Zhao, Xue, Jayarathna, Shishanthi, Turesson, Helle, Fält, Ann Sofie, Nestor, Gustav, Gonzalez, Matías Nicolás, Olsson, Niklas, Beganovic, Mirela, Hofvander, Per, Andersson, Roger, Anderson, Mariette
Format:	info:ar-repo/semantics/artículo
Language:	Inglés
Published:	Nature Publishing Group 2021
Subjects:	Papa Enzimas Almidón Amilosa Amilopectinia Potatoes Enzymes Genes Mutagenesis Starch Amilose Amylopectin
Online Access:	http://hdl.handle.net/20.500.12123/9235 https://www.nature.com/articles/s41598-021-83462-z https://doi.org/10.1038/s41598-021-83462-z