Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72%...

Descripción completa

Detalles Bibliográficos

Autores principales:	Zhao, Xue, Jayarathna, Shishanthi, Turesson, Helle, Fält, Ann Sofie, Nestor, Gustav, Gonzalez, Matías Nicolás, Olsson, Niklas, Beganovic, Mirela, Hofvander, Per, Andersson, Roger, Anderson, Mariette
Formato:	Artículo
Lenguaje:	Inglés
Publicado:	Nature Publishing Group 2021
Materias:	Papa Enzimas Almidón Amilosa Amilopectinia Potatoes Enzymes Genes Mutagenesis Starch Amilose Amylopectin
Acceso en línea:	http://hdl.handle.net/20.500.12123/9235 https://www.nature.com/articles/s41598-021-83462-z https://doi.org/10.1038/s41598-021-83462-z