Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit dur-ing the postharvest. Several studies have been dedicated to investigate the general chemical changes thatjustify the immediate reaction responsible for the induced tolerance; but just how primar...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2829 https://www.sciencedirect.com/science/article/pii/S0925521414002415 https://doi.org/10.1016/j.pmpp.2016.05.001 |
| _version_ | 1855035034004619264 |
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| author | Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina del Vecchio, Hernán A. Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Podesta, Florencio Esteban |
| author_browse | Bello, Fernando Cocco, Mariángeles Meier, Guillermo Enrique Moreno, Alejandra Soledad Perotti, Valeria Elisa Podesta, Florencio Esteban Tripodi, Karina Eva Josefina Vazquez, Daniel Eduardo del Vecchio, Hernán A. |
| author_facet | Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina del Vecchio, Hernán A. Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Podesta, Florencio Esteban |
| author_sort | Perotti, Valeria Elisa |
| collection | INTA Digital |
| description | Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit dur-ing the postharvest. Several studies have been dedicated to investigate the general chemical changes thatjustify the immediate reaction responsible for the induced tolerance; but just how primary metabolismand enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. Inthis work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during thepostharvest period after a heat treatment (HT) of 48 h at 37◦C. Enzymatic activity measurements indi-cated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylatingglyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthe-sis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would havean important role in sucrose accumulation. The pathway of carbon through glycolysis was affected bycold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase overthe PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxy-lase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes inresponse to HT, although some of them showed a marked descent along the cold storage. Proteomic stud-ies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and smallHSPs, completing the description of the main metabolic changes in this tissue. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2829 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28292019-03-19T13:04:22Z Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina del Vecchio, Hernán A. Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Podesta, Florencio Esteban Frutas Cítricas Naranja Dulce Glicólisis Almacenamiento en Frío Tratamiento Térmico Tecnología Postcosecha Postharvest Technology Heat Treatment Cold Storage Glycolysis Sweet Oranges Citrus Fruits Proteomics Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit dur-ing the postharvest. Several studies have been dedicated to investigate the general chemical changes thatjustify the immediate reaction responsible for the induced tolerance; but just how primary metabolismand enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. Inthis work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during thepostharvest period after a heat treatment (HT) of 48 h at 37◦C. Enzymatic activity measurements indi-cated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylatingglyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthe-sis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would havean important role in sucrose accumulation. The pathway of carbon through glycolysis was affected bycold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase overthe PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxy-lase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes inresponse to HT, although some of them showed a marked descent along the cold storage. Proteomic stud-ies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and smallHSPs, completing the description of the main metabolic changes in this tissue. EEA Concordia Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Moreno, Alejandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2018-07-19T15:16:52Z 2018-07-19T15:16:52Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2829 https://www.sciencedirect.com/science/article/pii/S0925521414002415 0925-5214 https://doi.org/10.1016/j.pmpp.2016.05.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Postharvest biology and technology 99 : 80–87. (2015) |
| spellingShingle | Frutas Cítricas Naranja Dulce Glicólisis Almacenamiento en Frío Tratamiento Térmico Tecnología Postcosecha Postharvest Technology Heat Treatment Cold Storage Glycolysis Sweet Oranges Citrus Fruits Proteomics Perotti, Valeria Elisa Moreno, Alejandra Soledad Tripodi, Karina Eva Josefina del Vecchio, Hernán A. Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Vazquez, Daniel Eduardo Podesta, Florencio Esteban Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
| title | Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
| title_full | Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
| title_fullStr | Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
| title_full_unstemmed | Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
| title_short | Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
| title_sort | biochemical characterization of the flavedo of heat treated valencia orange during postharvest cold storage |
| topic | Frutas Cítricas Naranja Dulce Glicólisis Almacenamiento en Frío Tratamiento Térmico Tecnología Postcosecha Postharvest Technology Heat Treatment Cold Storage Glycolysis Sweet Oranges Citrus Fruits Proteomics |
| url | http://hdl.handle.net/20.500.12123/2829 https://www.sciencedirect.com/science/article/pii/S0925521414002415 https://doi.org/10.1016/j.pmpp.2016.05.001 |
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