Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children

Objectives Provitamin A carotenoid biofortified “orange” maize has been developed as a vitamin A deficiency control strategy using selective breeding for the β-carotene hydroxylase-1 gene, which increases β-carotene, β-cryptoxanthin, and zeaxanthin in the endosperm. We conducted a cluster-randomized...

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Main Authors: Craft, Neal E., Palmer, Amanda, Schultze, Kerry, Chileshe, Justin, Barffour, Maxwell, Siamusantu, Ward, Klemm, Rolf, West, Keith
Format: Abstract
Language:Inglés
Published: Wiley 2017
Subjects:
Online Access:https://hdl.handle.net/10568/171052
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author Craft, Neal E.
Palmer, Amanda
Schultze, Kerry
Chileshe, Justin
Barffour, Maxwell
Siamusantu, Ward
Klemm, Rolf
West, Keith
author_browse Barffour, Maxwell
Chileshe, Justin
Craft, Neal E.
Klemm, Rolf
Palmer, Amanda
Schultze, Kerry
Siamusantu, Ward
West, Keith
author_facet Craft, Neal E.
Palmer, Amanda
Schultze, Kerry
Chileshe, Justin
Barffour, Maxwell
Siamusantu, Ward
Klemm, Rolf
West, Keith
author_sort Craft, Neal E.
collection Repository of Agricultural Research Outputs (CGSpace)
description Objectives Provitamin A carotenoid biofortified “orange” maize has been developed as a vitamin A deficiency control strategy using selective breeding for the β-carotene hydroxylase-1 gene, which increases β-carotene, β-cryptoxanthin, and zeaxanthin in the endosperm. We conducted a cluster-randomized trial to test the impact of orange maize consumption (a predominantly β-carotene-rich variety) on serum retinol concentration in rural Zambian children (4–8 y). In the present analysis, we tested the intervention’s impact on circulating carotenoids. Methods Children (n=1024, 4–8 y) were cluster-randomized to receive 200 g dry weight/d orange or conventional “white” maize prepared with standardized low-vitamin A relishes, 6 d/w for 6 m. The carotenoid profile was assessed in baseline and follow-up serum samples in a subset (n=321 white, n=358 orange) of children. We assessed differences in log-transformed carotenoids, expressed as geometric means and 95% confidence limits, accounting for cluster randomization. Results Baseline carotenoids did not differ between groups. There were no differences in lycopene and lutein between groups at follow-up. Other carotenoids increased significantly (p<0.001) in orange vs white groups following the six-month intervention: β-carotene [273 (254,292) vs 147 (135,160) nmol/L], α-carotene [19.7 (18.4,21.1) vs 10.6 (9.9,11.3) nmol/L], β-cryptoxanthin [36.1 (33.6,38.8) vs 12.6 (11.8,13.5) nmol/L], α-cryptoxanthin [18.7 (17.4,20.1) vs 8.9 (8.4,9.4) nmol/L], zeaxanthin [127 (119,135) vs 81 (75,88) nmol/L]. Conclusions Children regularly consuming a biofortified maize variety bred primarily for β-carotene had higher concentrations of other provitamin A carotenoids and zeaxanthin, suggesting the potential for health benefits beyond vitamin A deficiency control.
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spelling CGSpace1710522025-02-19T14:31:11Z Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children Craft, Neal E. Palmer, Amanda Schultze, Kerry Chileshe, Justin Barffour, Maxwell Siamusantu, Ward Klemm, Rolf West, Keith provitamins metabolism retinol maize food production carotenes children nutrition Objectives Provitamin A carotenoid biofortified “orange” maize has been developed as a vitamin A deficiency control strategy using selective breeding for the β-carotene hydroxylase-1 gene, which increases β-carotene, β-cryptoxanthin, and zeaxanthin in the endosperm. We conducted a cluster-randomized trial to test the impact of orange maize consumption (a predominantly β-carotene-rich variety) on serum retinol concentration in rural Zambian children (4–8 y). In the present analysis, we tested the intervention’s impact on circulating carotenoids. Methods Children (n=1024, 4–8 y) were cluster-randomized to receive 200 g dry weight/d orange or conventional “white” maize prepared with standardized low-vitamin A relishes, 6 d/w for 6 m. The carotenoid profile was assessed in baseline and follow-up serum samples in a subset (n=321 white, n=358 orange) of children. We assessed differences in log-transformed carotenoids, expressed as geometric means and 95% confidence limits, accounting for cluster randomization. Results Baseline carotenoids did not differ between groups. There were no differences in lycopene and lutein between groups at follow-up. Other carotenoids increased significantly (p<0.001) in orange vs white groups following the six-month intervention: β-carotene [273 (254,292) vs 147 (135,160) nmol/L], α-carotene [19.7 (18.4,21.1) vs 10.6 (9.9,11.3) nmol/L], β-cryptoxanthin [36.1 (33.6,38.8) vs 12.6 (11.8,13.5) nmol/L], α-cryptoxanthin [18.7 (17.4,20.1) vs 8.9 (8.4,9.4) nmol/L], zeaxanthin [127 (119,135) vs 81 (75,88) nmol/L]. Conclusions Children regularly consuming a biofortified maize variety bred primarily for β-carotene had higher concentrations of other provitamin A carotenoids and zeaxanthin, suggesting the potential for health benefits beyond vitamin A deficiency control. 2017-04 2025-01-29T12:57:39Z 2025-01-29T12:57:39Z Abstract https://hdl.handle.net/10568/171052 en Limited Access Wiley Craft, Neal E.; Palmer, Amanda; Schultze, Kerry; Chileshe, Justin; Barffour, Maxwell; Siamusantu, Ward; Klemm, Rolf; and West, Keith. 2017. Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children. FASEB Journal 31 (1): Supplement 786.17: https://doi.org/10.1096/fasebj.31.1_supplement.786.17
spellingShingle provitamins
metabolism
retinol
maize
food production
carotenes
children
nutrition
Craft, Neal E.
Palmer, Amanda
Schultze, Kerry
Chileshe, Justin
Barffour, Maxwell
Siamusantu, Ward
Klemm, Rolf
West, Keith
Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children
title Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children
title_full Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children
title_fullStr Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children
title_full_unstemmed Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children
title_short Impact of provitamin A biofortified maize consumption on carotenoid status of Zambian children
title_sort impact of provitamin a biofortified maize consumption on carotenoid status of zambian children
topic provitamins
metabolism
retinol
maize
food production
carotenes
children
nutrition
url https://hdl.handle.net/10568/171052
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