Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population
The Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different variants in SfCOL-wt a plaque assay was performed and ten distinct genotypes were identified (called A through J...
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| Format: | Artículo |
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Public Library of Science
2024
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| Online Access: | https://digital.csic.es/handle/10261/98750 http://hdl.handle.net/20.500.12324/40216 |
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| author | Barrera, Gloria Trevor, Williams Villamizar, Laura Caballero, Primitivo Oihane, Simón |
| author_browse | Barrera, Gloria Caballero, Primitivo Oihane, Simón Trevor, Williams Villamizar, Laura |
| author_facet | Barrera, Gloria Trevor, Williams Villamizar, Laura Caballero, Primitivo Oihane, Simón |
| author_sort | Barrera, Gloria |
| collection | Repositorio AGROSAVIA |
| description | The Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different variants in SfCOL-wt a plaque assay was performed and ten distinct genotypes were identified (called A through J). SfCOL-A was the most prevalent (71±9%) and showed a PstI restriction profile identical to that of SfCOL-wt. The remaining nine genotypes presented genomic deletions of 3.8-21.8 Kb, located between nucleotides 11.436 and 33.883 in the Nicaraguan genotype of SfMNPV (SfMNPV-B). These deletions affected the region between open reading frames (ORFs) sf20 and sf33. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of mixtures of OBs and co-occluded mixed genotype OBs were consistently reduced in twogenotype mixtures involving equal proportions of SfCOL-A and one of three deletion genotypes (SfCOL-C, -D or –F). Speed of kill and OB production were improved only when the certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any of the component genotypes or mixtures thereof. We conclude that the SfCOL-wt population is structured to maximize the production of OBs in each infected host. |
| format | Artículo |
| id | RepoAGROSAVIA40216 |
| institution | Corporación Colombiana de Investigación Agropecuaria |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | RepoAGROSAVIA402162024-10-17T03:00:57Z Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population Barrera, Gloria Trevor, Williams Villamizar, Laura Caballero, Primitivo Oihane, Simón Genética vegetal y fitomejoramiento - F30 Spodoptera frugiperda Zea mays Genotipado Producción Transitorios http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_a29eab99 http://aims.fao.org/aos/agrovoc/c_6200 The Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different variants in SfCOL-wt a plaque assay was performed and ten distinct genotypes were identified (called A through J). SfCOL-A was the most prevalent (71±9%) and showed a PstI restriction profile identical to that of SfCOL-wt. The remaining nine genotypes presented genomic deletions of 3.8-21.8 Kb, located between nucleotides 11.436 and 33.883 in the Nicaraguan genotype of SfMNPV (SfMNPV-B). These deletions affected the region between open reading frames (ORFs) sf20 and sf33. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of mixtures of OBs and co-occluded mixed genotype OBs were consistently reduced in twogenotype mixtures involving equal proportions of SfCOL-A and one of three deletion genotypes (SfCOL-C, -D or –F). Speed of kill and OB production were improved only when the certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any of the component genotypes or mixtures thereof. We conclude that the SfCOL-wt population is structured to maximize the production of OBs in each infected host. Departamento Administrativo de Ciencia, Tecnología e Innovación - COLCIENCIAS Maíz-Zea mays 2024-10-16T15:43:45Z 2024-10-16T15:43:45Z 2013-06 2013 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://digital.csic.es/handle/10261/98750 1027-3115 http://hdl.handle.net/20.500.12324/40216 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Red de Bibliotecas y Archivos del CSIC 1 1 1 5 Barrera, G., Simón, O., Villamizar, L., Williams, T. & Caballero P. 2011: Spodoptera frugiperda multiple nucleopolyhedrovirus as a potential biological insecticide: Genetic and phenotypic comparison of field isolates from Colombia. Biol Control 58: 113-120 Crawley, M. J. 1993: GLIM for ecologists. Blackwell Scientific Publications, Oxford; Boston. Harrison, R. L., Puttler, B., Popham, H. J. 2008: Genomic sequence analysis of a fastkilling isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus. J Gen Virol 89: 775-790. LeOra-Software 1987: POLO-PC a user`s guide to Probit or Logit analysis. Berkeley, CA. Muñoz D., Castillejo, J. I., Caballero, P. 1998: Naturally occurring deletion mutants are parasitic genotypes in a wild-type nucleopolyhedrovirus population of Spodoptera exigua. Appl Environ Microbiol 64: 4372-4377. Simón, O., Williams, T., López-Ferber, M. & Caballero, P. 2004: Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: High prevalence of deletion genotypes. Appl. Environ. Microbiol. 70, 5579-88. Simón, O., Palma, L., Beperet, I., Muñoz, D., Williams, T., Caballero, P. & LópezFerber, M. 2011: Sequence comparison between three geographically distinct Spodoptera fugiperda multiple nucleopolyhedrovirus isolates detecting positively selected genes. J. Invertebr. Pathol. 107:33-41. Simón, O., Williams, T., López-Ferber, M. & Caballero, P. 2005: Functional importance of deletion mutant genotypes in an insect nucleopolyhedrovirus population. Appl Environ Microbiol 71: 4254-4262. Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf Colombia Public Library of Science San Francisco (Estados Unidos) Red de Bibliotecas y Archivos del CSIC; (2013): Red de Bibliotecas y Archivos del CSIC (Junio);p. 1 - 5. |
| spellingShingle | Genética vegetal y fitomejoramiento - F30 Spodoptera frugiperda Zea mays Genotipado Producción Transitorios http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_a29eab99 http://aims.fao.org/aos/agrovoc/c_6200 Barrera, Gloria Trevor, Williams Villamizar, Laura Caballero, Primitivo Oihane, Simón Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population |
| title | Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population |
| title_full | Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population |
| title_fullStr | Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population |
| title_full_unstemmed | Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population |
| title_short | Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population |
| title_sort | deletion genotypes reduce occlusion body potency but increase occlusion body production in a colombian spodoptera frugiperda nucleopolyhedrovirus population |
| topic | Genética vegetal y fitomejoramiento - F30 Spodoptera frugiperda Zea mays Genotipado Producción Transitorios http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_a29eab99 http://aims.fao.org/aos/agrovoc/c_6200 |
| url | https://digital.csic.es/handle/10261/98750 http://hdl.handle.net/20.500.12324/40216 |
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