Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt

Bacillus velezensis Bs006 has shown antagonistic activity on Fusarium oxysporum f. sp. physali and biocontrol activity against Fusarium wilt (FW) in golden berry (Physalis peruviana). We hypothesized that strain Bs006 has the ability to synthesize antimicrobial cyclic lipopeptides (CLPs) like other...

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Main Authors:	Ongena, Marc, Moreno Velandia, Carlos Andrés, Cotes Prada, Alba Marina
Format:	article
Language:	Inglés
Published:	Biological Control and Microbial Ecology 2024
Subjects:	Fertilización - F04 Control biológico Physalis peruviana Fungicidas Frutales http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_26616 http://aims.fao.org/aos/agrovoc/c_3146
Online Access:	https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-01-21-0001-R http://hdl.handle.net/20.500.12324/38911

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spelling	RepoAGROSAVIA389112024-02-22T03:02:03Z Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt Ongena, Marc Moreno Velandia, Carlos Andrés Cotes Prada, Alba Marina Fertilización - F04 Control biológico Physalis peruviana Fungicidas Frutales http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_26616 http://aims.fao.org/aos/agrovoc/c_3146 Bacillus velezensis Bs006 has shown antagonistic activity on Fusarium oxysporum f. sp. physali and biocontrol activity against Fusarium wilt (FW) in golden berry (Physalis peruviana). We hypothesized that strain Bs006 has the ability to synthesize antimicrobial cyclic lipopeptides (CLPs) like other members of the same species. However, if so, the real effects of CLPs on F. oxysporum f. sp. physali and their potential as a biocontrol tool against Physalis-FW have not been elucidated. In this study the CLPs profile of Bs006 in liquid culture and antagonist–plant–pathogen interactions were characterized. Also, the potential effects of supernatant free of bacteria against F. oxysporum f. sp. physali and FW were explored and compared with the effects of pure CLPs. Ultraperformance liquid chromatography–electrospray ionization–mass spectrometry analysis revealed the capacity of Bs006 to synthesize homologous compounds of iturins, surfactins, and fengycins in liquid culture and on the inhibition zone against F. oxysporum f. sp. physali in dual confrontation tests. Bs006 supernatant reduced the germination and growth of F. oxysporum f. sp. physali and caused vacuolization, swelling, and lysis of F. oxysporum f. sp. physali cells in a concentration-dependent manner. Pure fengycins affected the development of F. oxysporum f. sp. physali from 11 mg/liter and iturins from 21 mg/liter. In a gnotobiotic system, Bs006 colonized the root surface of golden berry, inhibited the growth of F. oxysporum f. sp. physali, and produced CLPs. Individual application of Bs006 and supernatant protected the plants from F. oxysporum f. sp. physali infections by 37 to 53%, respectively. Meanwhile, fengycins reduced the disease progress by 39%. These results suggest further studies to select an optimum combination of Bs006 and supernatant or CLPs, which might be a good option as biofungicide against F. oxysporum f. sp. physali. Uchuva-Physalis peruviana L. 2024-02-21T16:12:51Z 2024-02-21T16:12:51Z 2021-05-19 2021 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-01-21-0001-R 0031-949X http://hdl.handle.net/20.500.12324/38911 10.1094/PHYTO-01-21-0001-R reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Phytopathology® 111 12 1 11 Attribution-ShareAlike 4.0 International http://creativecommons.org/licenses/by-sa/4.0/ application/pdf application/pdf Biological Control and Microbial Ecology Si lugar de publicación Phytopathology®; Vol 111 No.12 (2021):Phytopathology® Vol 111 No.12 (mayo);p. 1 -11.
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Fertilización - F04 Control biológico Physalis peruviana Fungicidas Frutales http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_26616 http://aims.fao.org/aos/agrovoc/c_3146
spellingShingle	Fertilización - F04 Control biológico Physalis peruviana Fungicidas Frutales http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_26616 http://aims.fao.org/aos/agrovoc/c_3146 Ongena, Marc Moreno Velandia, Carlos Andrés Cotes Prada, Alba Marina Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt
description	Bacillus velezensis Bs006 has shown antagonistic activity on Fusarium oxysporum f. sp. physali and biocontrol activity against Fusarium wilt (FW) in golden berry (Physalis peruviana). We hypothesized that strain Bs006 has the ability to synthesize antimicrobial cyclic lipopeptides (CLPs) like other members of the same species. However, if so, the real effects of CLPs on F. oxysporum f. sp. physali and their potential as a biocontrol tool against Physalis-FW have not been elucidated. In this study the CLPs profile of Bs006 in liquid culture and antagonist–plant–pathogen interactions were characterized. Also, the potential effects of supernatant free of bacteria against F. oxysporum f. sp. physali and FW were explored and compared with the effects of pure CLPs. Ultraperformance liquid chromatography–electrospray ionization–mass spectrometry analysis revealed the capacity of Bs006 to synthesize homologous compounds of iturins, surfactins, and fengycins in liquid culture and on the inhibition zone against F. oxysporum f. sp. physali in dual confrontation tests. Bs006 supernatant reduced the germination and growth of F. oxysporum f. sp. physali and caused vacuolization, swelling, and lysis of F. oxysporum f. sp. physali cells in a concentration-dependent manner. Pure fengycins affected the development of F. oxysporum f. sp. physali from 11 mg/liter and iturins from 21 mg/liter. In a gnotobiotic system, Bs006 colonized the root surface of golden berry, inhibited the growth of F. oxysporum f. sp. physali, and produced CLPs. Individual application of Bs006 and supernatant protected the plants from F. oxysporum f. sp. physali infections by 37 to 53%, respectively. Meanwhile, fengycins reduced the disease progress by 39%. These results suggest further studies to select an optimum combination of Bs006 and supernatant or CLPs, which might be a good option as biofungicide against F. oxysporum f. sp. physali.
format	article
author	Ongena, Marc Moreno Velandia, Carlos Andrés Cotes Prada, Alba Marina
author_facet	Ongena, Marc Moreno Velandia, Carlos Andrés Cotes Prada, Alba Marina
author_sort	Ongena, Marc
title	Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt
title_short	Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt
title_full	Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt
title_fullStr	Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt
title_full_unstemmed	Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt
title_sort	effects of fengycins and iturins on fusarium oxysporum f. sp. physali and root colonization by bacillus velezensis bs006 protect golden berry against vascular wilt
publisher	Biological Control and Microbial Ecology
publishDate	2024
url	https://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-01-21-0001-R http://hdl.handle.net/20.500.12324/38911
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_version_	1808107148986548224

Effects of Fengycins and Iturins on Fusarium oxysporum f. sp. physali and Root Colonization by Bacillus velezensis Bs006 Protect Golden Berry Against Vascular Wilt

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