Detection of Mycobacterium bovis in Bovine and Bubaline tissues using Nested-PCR for TbD1

In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA sa...

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Main Authors:	Araújo, Cristina Pires de, Osório, Ana Luiza Alves Rosa, Jorge, Klaudia dos Santos Goncalves, Ramos, Carlos Alberto N., Filho, Antonio Francisco S., Vidal, Carlos Eugênio Soto, Roxo, Eliana, Nishibe, Christiane, Almeida Junior, Nalvo Franco de, Júnior, Antônio A. F., Silva, Marcio Roberto, Neto, Jose Diomedes Barbosa, Cerqueira, Valíria Duarte, Zumarraga, Martin Jose, Araujo, Flabio Ribeiro de
Format:	info:ar-repo/semantics/artículo
Language:	Inglés
Published:	PLOS 2019
Subjects:	Mycobacterium Bovis Tejidos Animales Ganado Bovino ADN Tuberculosis PCR Animal Tissues Cattle DNA TbD1 Polymerase Chain Reaction
Online Access:	http://hdl.handle.net/20.500.12123/4855 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091023 https://doi.org/10.1371/journal.pone.0091023