Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0925521411001323 http://hdl.handle.net/20.500.12123/4545 https://doi.org/10.1016/j.postharvbio.2011.05.015 |
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| author | Perotti, Valeria Elisa del Vecchio, Hernán A. Sansevich, Alina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Garran, Sergio Mario Anderson, Catalina Margarita Vazquez, Daniel Eduardo Podesta, Florencio Esteban |
| author_browse | Anderson, Catalina Margarita Bello, Fernando Cocco, Mariángeles Garran, Sergio Mario Meier, Guillermo Enrique Perotti, Valeria Elisa Podesta, Florencio Esteban Sansevich, Alina Vazquez, Daniel Eduardo del Vecchio, Hernán A. |
| author_facet | Perotti, Valeria Elisa del Vecchio, Hernán A. Sansevich, Alina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Garran, Sergio Mario Anderson, Catalina Margarita Vazquez, Daniel Eduardo Podesta, Florencio Esteban |
| author_sort | Perotti, Valeria Elisa |
| collection | INTA Digital |
| description | Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. Twenty eight different proteins in 40 spots among the 61 analyzed were identified. Functional classification showed that the main affected categories were “Cell rescue, defense, and virulence” and “Metabolism”; while 20% were found to be storage proteins with the rest related to minor categories. Activity of antioxidant enzymes was also evaluated. The most conspicuous change was an increase in superoxide dismutase in both the flavedo and juice sacs. Peroxidases increased in flavedo but decreased in sacs while alcohol dehydrogenase increased in treated flavedo, but not in sacs. Other fermentative enzymes were not affected. Metabolite content showed a higher concentration of sugars in heat treated fruit, while the main acids were slightly or not affected. These results are consistent with the development of a lower degree of fruit susceptibility to fungal pathogens, thus explaining the maintenance of postharvest quality. |
| format | Artículo |
| id | INTA4545 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA45452019-03-07T14:49:52Z Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage Perotti, Valeria Elisa del Vecchio, Hernán A. Sansevich, Alina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Garran, Sergio Mario Anderson, Catalina Margarita Vazquez, Daniel Eduardo Podesta, Florencio Esteban Naranja Dulce Variedades Tratamiento Térmico Citrus Tecnología Postcosecha Sweet Oranges Varieties Heat Treatment Postharvest Technology Proteomics Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. Twenty eight different proteins in 40 spots among the 61 analyzed were identified. Functional classification showed that the main affected categories were “Cell rescue, defense, and virulence” and “Metabolism”; while 20% were found to be storage proteins with the rest related to minor categories. Activity of antioxidant enzymes was also evaluated. The most conspicuous change was an increase in superoxide dismutase in both the flavedo and juice sacs. Peroxidases increased in flavedo but decreased in sacs while alcohol dehydrogenase increased in treated flavedo, but not in sacs. Other fermentative enzymes were not affected. Metabolite content showed a higher concentration of sugars in heat treated fruit, while the main acids were slightly or not affected. These results are consistent with the development of a lower degree of fruit susceptibility to fungal pathogens, thus explaining the maintenance of postharvest quality. EEA Concordia Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Sansevich, Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Garran, Sergio Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Anderson, Catalina Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2019-03-07T14:45:35Z 2019-03-07T14:45:35Z 2011-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0925521411001323 http://hdl.handle.net/20.500.12123/4545 0925-5214 https://doi.org/10.1016/j.postharvbio.2011.05.015 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Postharvest Biology and Technology 62 (2) : 97-114 (November 2011) |
| spellingShingle | Naranja Dulce Variedades Tratamiento Térmico Citrus Tecnología Postcosecha Sweet Oranges Varieties Heat Treatment Postharvest Technology Proteomics Perotti, Valeria Elisa del Vecchio, Hernán A. Sansevich, Alina Meier, Guillermo Enrique Bello, Fernando Cocco, Mariángeles Garran, Sergio Mario Anderson, Catalina Margarita Vazquez, Daniel Eduardo Podesta, Florencio Esteban Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage |
| title | Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage |
| title_full | Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage |
| title_fullStr | Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage |
| title_full_unstemmed | Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage |
| title_short | Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage |
| title_sort | proteomic metabalomic and biochemical analysis of heat treated valencia oranges during storage |
| topic | Naranja Dulce Variedades Tratamiento Térmico Citrus Tecnología Postcosecha Sweet Oranges Varieties Heat Treatment Postharvest Technology Proteomics |
| url | https://www.sciencedirect.com/science/article/pii/S0925521411001323 http://hdl.handle.net/20.500.12123/4545 https://doi.org/10.1016/j.postharvbio.2011.05.015 |
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