Rapid autographic method for detection of enzymatic browning inhibitors based on enzyme immobilization
This work describes a TLC-coupled bioautographic assay suitable for the separation and detection of apple polyphenol oxidase (PPO) inhibitors from natural extracts. PPO was immobilised in agar containing L-DOPA as substrate and 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) to enhance c...
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/2935 https://www.sciencedirect.com/science/article/pii/S0308814618311646 https://doi.org/10.1016/j.foodchem.2018.07.025 |
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