Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected w...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2827 https://doi.org/10.1016/j.pmpp.2016.05.001 |
| Sumario: | Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants
were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to
expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they
showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the
recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with
Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific
action of 24K-derived hairpin over CPsV multiplication. |
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