Cover Image
QR Code

Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing

Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected w...

Full description

Main Authors: Reyes Martinez, Carina Andrea, de Francesco, Agustina, Ocolotobiche, Eliana Evelina, Costa, Norma Beatriz, Garcia, Maria Laura
Format: Artículo
Language:Inglés
Published: 2018
Subjects:
Citrus Sinensis
Infección
Virus de las Plantas
Plantas Transgénicas
Naranja Dulce
ARN
RNA
Sweet Oranges
Transgenic Plants
Plant Viruses
Infection
Citrus Psorosis Virus
Hypersusceptibility
Hairpin-RNA
Virus de la Psoriasis de los Cítricos
Online Access:http://hdl.handle.net/20.500.12123/2827
https://doi.org/10.1016/j.pmpp.2016.05.001
Summary:Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific action of 24K-derived hairpin over CPsV multiplication.

Similar Items