Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing

Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected w...

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Main Authors: Reyes Martinez, Carina Andrea, de Francesco, Agustina, Ocolotobiche, Eliana Evelina, Costa, Norma Beatriz, Garcia, Maria Laura
Format: Artículo
Language:Inglés
Published: 2018
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/2827
https://doi.org/10.1016/j.pmpp.2016.05.001
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author Reyes Martinez, Carina Andrea
de Francesco, Agustina
Ocolotobiche, Eliana Evelina
Costa, Norma Beatriz
Garcia, Maria Laura
author_browse Costa, Norma Beatriz
Garcia, Maria Laura
Ocolotobiche, Eliana Evelina
Reyes Martinez, Carina Andrea
de Francesco, Agustina
author_facet Reyes Martinez, Carina Andrea
de Francesco, Agustina
Ocolotobiche, Eliana Evelina
Costa, Norma Beatriz
Garcia, Maria Laura
author_sort Reyes Martinez, Carina Andrea
collection INTA Digital
description Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific action of 24K-derived hairpin over CPsV multiplication.
format Artículo
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2018
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spelling INTA28272018-07-19T14:28:53Z Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing Reyes Martinez, Carina Andrea de Francesco, Agustina Ocolotobiche, Eliana Evelina Costa, Norma Beatriz Garcia, Maria Laura Citrus Sinensis Infección Virus de las Plantas Plantas Transgénicas Naranja Dulce ARN RNA Sweet Oranges Transgenic Plants Plant Viruses Infection Citrus Sinensis Citrus Psorosis Virus Hypersusceptibility Hairpin-RNA Virus de la Psoriasis de los Cítricos Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific action of 24K-derived hairpin over CPsV multiplication. EEA Concordia Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Ocolotobiche, Eliana Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Costa, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina 2018-07-19T14:20:28Z 2018-07-19T14:20:28Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2827 0885-5765 https://doi.org/10.1016/j.pmpp.2016.05.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Physiological and molecular plant pathology 94 : 149-155. (2016)
spellingShingle Citrus Sinensis
Infección
Virus de las Plantas
Plantas Transgénicas
Naranja Dulce
ARN
RNA
Sweet Oranges
Transgenic Plants
Plant Viruses
Infection
Citrus Sinensis
Citrus Psorosis Virus
Hypersusceptibility
Hairpin-RNA
Virus de la Psoriasis de los Cítricos
Reyes Martinez, Carina Andrea
de Francesco, Agustina
Ocolotobiche, Eliana Evelina
Costa, Norma Beatriz
Garcia, Maria Laura
Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
title Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
title_full Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
title_fullStr Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
title_full_unstemmed Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
title_short Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing
title_sort uncontrolled citrus psorosis virus infection in citrus sinensis transgenic plants expressing a viral 24k derived hairpin that does not trigger rna silencing
topic Citrus Sinensis
Infección
Virus de las Plantas
Plantas Transgénicas
Naranja Dulce
ARN
RNA
Sweet Oranges
Transgenic Plants
Plant Viruses
Infection
Citrus Sinensis
Citrus Psorosis Virus
Hypersusceptibility
Hairpin-RNA
Virus de la Psoriasis de los Cítricos
url http://hdl.handle.net/20.500.12123/2827
https://doi.org/10.1016/j.pmpp.2016.05.001
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