Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus
We aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the β-adrenergic octopamine receptor gene (RmβAOR). We established six populations of Rhipicephalus microplus...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
National Academy of Sciences
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/20165 https://www.pnas.org/doi/full/10.1073/pnas.1309072110 https://doi.org/10.1073/pnas.1309072110 |
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| author | Corley, Sean W. Jonsson, Nicholas N. Piper, Emily K. Cutulle, Christian Stear, Michael J. Seddon, Jennifer M. |
| author_browse | Corley, Sean W. Cutulle, Christian Jonsson, Nicholas N. Piper, Emily K. Seddon, Jennifer M. Stear, Michael J. |
| author_facet | Corley, Sean W. Jonsson, Nicholas N. Piper, Emily K. Cutulle, Christian Stear, Michael J. Seddon, Jennifer M. |
| author_sort | Corley, Sean W. |
| collection | INTA Digital |
| description | We aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the β-adrenergic octopamine receptor gene (RmβAOR). We established six populations of Rhipicephalus microplus ticks in similar paddocks by the admixture of ticks from strains known to be susceptible and resistant to amitraz and synthetic pyrethroids. Each population was managed using one of three acaricide treatment regimes: always amitraz, always spinosad, or rotation between amitraz and spinosad. We used microsatellites to elucidate population structure over time, an SNP in the para-sodium channel gene previously demonstrated to confer resistance to synthetic pyrethroids to quantify changes in resistance to synthetic pyrethroids over time, and a nonsynonymous SNP in the RmβAOR, a gene that we proposed to confer resistance to amitraz, to determine whether selection with amitraz increased the frequency of this mutation. The study showed panmixia of the two strains and that selection of ticks with amitraz increased the frequency of the RmβAOR mutation while increasing the prevalence of amitraz-resistance. We conclude that polymorphisms in the RmβAOR gene are likely to confer resistance to amitraz. |
| format | Artículo |
| id | INTA20165 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| record_format | dspace |
| spelling | INTA201652024-11-07T16:15:03Z Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus Corley, Sean W. Jonsson, Nicholas N. Piper, Emily K. Cutulle, Christian Stear, Michael J. Seddon, Jennifer M. Mutation Amitraz Pesticide Resistance Cattle Mutación Resistencia a los Plaguicidas Ganado Bovino Rhipicephalus Garrapatas Ticks We aimed to describe the evolution of resistance to amitraz in Rhipicephalus microplus in the field and to test the association between amitraz resistance and the frequency of a mutation in the β-adrenergic octopamine receptor gene (RmβAOR). We established six populations of Rhipicephalus microplus ticks in similar paddocks by the admixture of ticks from strains known to be susceptible and resistant to amitraz and synthetic pyrethroids. Each population was managed using one of three acaricide treatment regimes: always amitraz, always spinosad, or rotation between amitraz and spinosad. We used microsatellites to elucidate population structure over time, an SNP in the para-sodium channel gene previously demonstrated to confer resistance to synthetic pyrethroids to quantify changes in resistance to synthetic pyrethroids over time, and a nonsynonymous SNP in the RmβAOR, a gene that we proposed to confer resistance to amitraz, to determine whether selection with amitraz increased the frequency of this mutation. The study showed panmixia of the two strains and that selection of ticks with amitraz increased the frequency of the RmβAOR mutation while increasing the prevalence of amitraz-resistance. We conclude that polymorphisms in the RmβAOR gene are likely to confer resistance to amitraz. Instituto de Patobiología Fil: Corley, Sean W. University of Queensland. School of Veterinary Science; Australia Fil: Jonsson, Nicholas N. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido Fil: Piper, Emily K. University of Queensland. School of Veterinary Science; Australia Fil: Cutulle, Christian. University of Queensland. School of Veterinary Science; Australia Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Stear, Michael J. University of Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido Fil: Seddon, Jennifer M. University of Queensland. School of Veterinary Science; Australia 2024-11-07T16:06:25Z 2024-11-07T16:06:25Z 2013-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20165 https://www.pnas.org/doi/full/10.1073/pnas.1309072110 1091-6490 https://doi.org/10.1073/pnas.1309072110 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf National Academy of Sciences Proceedings of the National Academy of Sciences 110 (42) : 16772-16777 (Octubre 2013) |
| spellingShingle | Mutation Amitraz Pesticide Resistance Cattle Mutación Resistencia a los Plaguicidas Ganado Bovino Rhipicephalus Garrapatas Ticks Corley, Sean W. Jonsson, Nicholas N. Piper, Emily K. Cutulle, Christian Stear, Michael J. Seddon, Jennifer M. Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus |
| title | Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus |
| title_full | Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus |
| title_fullStr | Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus |
| title_full_unstemmed | Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus |
| title_short | Mutation in the RmβAOR gene is associated with amitraz resistance in the cattle tick Rhipicephalus microplus |
| title_sort | mutation in the rmβaor gene is associated with amitraz resistance in the cattle tick rhipicephalus microplus |
| topic | Mutation Amitraz Pesticide Resistance Cattle Mutación Resistencia a los Plaguicidas Ganado Bovino Rhipicephalus Garrapatas Ticks |
| url | http://hdl.handle.net/20.500.12123/20165 https://www.pnas.org/doi/full/10.1073/pnas.1309072110 https://doi.org/10.1073/pnas.1309072110 |
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