Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher value products, like whey protein hydrolysates. Proteases (EC: 3.4) represent...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/18638 https://link.springer.com/article/10.1007/s00253-023-12591-4 https://doi.org/10.1007/s00253-023-12591-4 |
| _version_ | 1855486214030753792 |
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| author | Irazoqui, Jose Matias Eberhardt, María Florencia Santiago, Gonzalo Manuel Amadio, Ariel |
| author_browse | Amadio, Ariel Eberhardt, María Florencia Irazoqui, Jose Matias Santiago, Gonzalo Manuel |
| author_facet | Irazoqui, Jose Matias Eberhardt, María Florencia Santiago, Gonzalo Manuel Amadio, Ariel |
| author_sort | Irazoqui, Jose Matias |
| collection | INTA Digital |
| description | Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher value products, like whey protein hydrolysates. Proteases (EC: 3.4) represent a large segment of industrial enzymes, since they are used in several industries, including food. In this work, we describe three novel enzymes identified using a metagenomic approach. Metagenomic DNA from dairy industry stabilization ponds were sequenced, and the predicted genes were compared against the MEROPS database, focusing on families commercially used to produce whey protein hydrolysates. From a total of 849 candidates, 10 were selected for cloning and expression and three showed activities with both the chromogenic substrate, azocasein, and whey proteins. Particularly, Pr05, an enzyme from the yet uncultured phylum Patescibacteria, showed activity that is comparable to a commercial protease. All these novel enzymes could represent an alternative for dairy industries to produce value-added products from industrial by-products. |
| format | Artículo |
| id | INTA18638 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA186382024-07-24T13:05:53Z Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds Irazoqui, Jose Matias Eberhardt, María Florencia Santiago, Gonzalo Manuel Amadio, Ariel Proteases Metagenomics Whey Protein Ponds Proteasa Metagenómica Proteínas de Suero de Leche Estanque Whey Protein Hydrolysates Bioprospection Hidrolizados de Proteína de Suero Bioprospección Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher value products, like whey protein hydrolysates. Proteases (EC: 3.4) represent a large segment of industrial enzymes, since they are used in several industries, including food. In this work, we describe three novel enzymes identified using a metagenomic approach. Metagenomic DNA from dairy industry stabilization ponds were sequenced, and the predicted genes were compared against the MEROPS database, focusing on families commercially used to produce whey protein hydrolysates. From a total of 849 candidates, 10 were selected for cloning and expression and three showed activities with both the chromogenic substrate, azocasein, and whey proteins. Particularly, Pr05, an enzyme from the yet uncultured phylum Patescibacteria, showed activity that is comparable to a commercial protease. All these novel enzymes could represent an alternative for dairy industries to produce value-added products from industrial by-products. ANPCyT PICT 2017–0191, ASACTeI IO-2017–00172 EEA Rafaela Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Eberhardt, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Eberhardt, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Santiago, Gonzalo Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Santiago, Gonzalo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina 2024-07-24T12:39:18Z 2024-07-24T12:39:18Z 2023-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18638 https://link.springer.com/article/10.1007/s00253-023-12591-4 1432-0614 (electronic) 0175-7598 https://doi.org/10.1007/s00253-023-12591-4 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I106-001, Estudios metagenómicos en animales y medio ambiente para modular la microbiota, desarrollar probióticos y mitigar el impacto ambiental de la producción pecuaria info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repograntAgreement/INTA/2019-PE-E7-I150-001, Aprovechamiento de residuos, descartes y subproductos agroalimentarios y agropecuarios: tecnologías para la obtención de alimentos y bioproductos para cadenas productivas info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Applied Microbiology and Biotechnology 107 : 4291-4300 (2023) |
| spellingShingle | Proteases Metagenomics Whey Protein Ponds Proteasa Metagenómica Proteínas de Suero de Leche Estanque Whey Protein Hydrolysates Bioprospection Hidrolizados de Proteína de Suero Bioprospección Irazoqui, Jose Matias Eberhardt, María Florencia Santiago, Gonzalo Manuel Amadio, Ariel Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds |
| title | Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds |
| title_full | Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds |
| title_fullStr | Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds |
| title_full_unstemmed | Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds |
| title_short | Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds |
| title_sort | characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds |
| topic | Proteases Metagenomics Whey Protein Ponds Proteasa Metagenómica Proteínas de Suero de Leche Estanque Whey Protein Hydrolysates Bioprospection Hidrolizados de Proteína de Suero Bioprospección |
| url | http://hdl.handle.net/20.500.12123/18638 https://link.springer.com/article/10.1007/s00253-023-12591-4 https://doi.org/10.1007/s00253-023-12591-4 |
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