Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation
Cassava (Manihot esculenta Crantz) biofortification with provitamin A carotenoids is an ongoing process that aims to alleviate vitamin A deficiency. The moderate content of provitamin A carotenoids achieved so far limits the contribution to providing adequate dietary vitamin A levels. Strategies to...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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2022
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| Online Access: | https://hdl.handle.net/10568/128585 |
| _version_ | 1855525221745819648 |
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| author | Jaramillo, Angélica M. Sierra, Santiago Chavarriaga-Aguirre, Paul Castillo, Diana Katherine Gkanogiannis, Anestis Becerra Lopez-Lavalle, Luis Augusto Arciniegas Vega, Juan Pablo Sun, Tianhu Li, Li Welsch, Ralf Boy, Erick Álvarez, Daniel |
| author_browse | Arciniegas Vega, Juan Pablo Becerra Lopez-Lavalle, Luis Augusto Boy, Erick Castillo, Diana Katherine Chavarriaga-Aguirre, Paul Gkanogiannis, Anestis Jaramillo, Angélica M. Li, Li Sierra, Santiago Sun, Tianhu Welsch, Ralf Álvarez, Daniel |
| author_facet | Jaramillo, Angélica M. Sierra, Santiago Chavarriaga-Aguirre, Paul Castillo, Diana Katherine Gkanogiannis, Anestis Becerra Lopez-Lavalle, Luis Augusto Arciniegas Vega, Juan Pablo Sun, Tianhu Li, Li Welsch, Ralf Boy, Erick Álvarez, Daniel |
| author_sort | Jaramillo, Angélica M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta Crantz) biofortification with provitamin A carotenoids is an ongoing process that aims to alleviate vitamin A deficiency. The moderate content of provitamin A carotenoids achieved so far limits the contribution to providing adequate dietary vitamin A levels. Strategies to increase carotenoid content focused on genes from the carotenoids biosynthesis pathway. In recent years, special emphasis was given to ORANGE protein (OR), which promotes the accumulation of carotenoids and their stability in several plants. The aim of this work was to identify, characterize and investigate the role of OR in the biosynthesis and stabilization of carotenoids in cassava and its relationship with phytoene synthase (PSY), the rate-limiting enzyme of the carotenoids biosynthesis pathway. Gene and protein characterization of OR, expression levels, protein amounts and carotenoids levels were evaluated in roots of one white (60444) and two yellow cassava cultivars (GM5309-57 and GM3736-37). Four OR variants were found in yellow cassava roots. Although comparable expression was found for three variants, significantly higher OR protein amounts were observed in the yellow varieties. In contrast, cassava PSY1 expression was significantly higher in the yellow cultivars, but PSY protein amount did not vary. Furthermore, we evaluated whether expression of one of the variants, MeOR_X1, affected carotenoid accumulation in cassava Friable Embryogenic Callus (FEC). Overexpression of maize PSY1 alone resulted in carotenoids accumulation and induced crystal formation. Co-expression with MeOR_X1 led to greatly increase of carotenoids although PSY1 expression was high in the co-expressed FEC. Our data suggest that posttranslational mechanisms controlling OR and PSY protein stability contribute to higher carotenoid levels in yellow cassava. Moreover, we showed that cassava FEC can be used to study the efficiency of single and combinatorial gene expression in increasing the carotenoid content prior to its application for the generation of biofortified cassava with enhanced carotenoids levels. |
| format | Journal Article |
| id | CGSpace128585 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| record_format | dspace |
| spelling | CGSpace1285852025-11-12T04:47:11Z Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation Jaramillo, Angélica M. Sierra, Santiago Chavarriaga-Aguirre, Paul Castillo, Diana Katherine Gkanogiannis, Anestis Becerra Lopez-Lavalle, Luis Augusto Arciniegas Vega, Juan Pablo Sun, Tianhu Li, Li Welsch, Ralf Boy, Erick Álvarez, Daniel provitamins carotenoids protein requirements provitaminas carotenoides requerimientos proteicos vitamin a biofortification health retinol nutrition vitamin deficiencies cassava diet public health Cassava (Manihot esculenta Crantz) biofortification with provitamin A carotenoids is an ongoing process that aims to alleviate vitamin A deficiency. The moderate content of provitamin A carotenoids achieved so far limits the contribution to providing adequate dietary vitamin A levels. Strategies to increase carotenoid content focused on genes from the carotenoids biosynthesis pathway. In recent years, special emphasis was given to ORANGE protein (OR), which promotes the accumulation of carotenoids and their stability in several plants. The aim of this work was to identify, characterize and investigate the role of OR in the biosynthesis and stabilization of carotenoids in cassava and its relationship with phytoene synthase (PSY), the rate-limiting enzyme of the carotenoids biosynthesis pathway. Gene and protein characterization of OR, expression levels, protein amounts and carotenoids levels were evaluated in roots of one white (60444) and two yellow cassava cultivars (GM5309-57 and GM3736-37). Four OR variants were found in yellow cassava roots. Although comparable expression was found for three variants, significantly higher OR protein amounts were observed in the yellow varieties. In contrast, cassava PSY1 expression was significantly higher in the yellow cultivars, but PSY protein amount did not vary. Furthermore, we evaluated whether expression of one of the variants, MeOR_X1, affected carotenoid accumulation in cassava Friable Embryogenic Callus (FEC). Overexpression of maize PSY1 alone resulted in carotenoids accumulation and induced crystal formation. Co-expression with MeOR_X1 led to greatly increase of carotenoids although PSY1 expression was high in the co-expressed FEC. Our data suggest that posttranslational mechanisms controlling OR and PSY protein stability contribute to higher carotenoid levels in yellow cassava. Moreover, we showed that cassava FEC can be used to study the efficiency of single and combinatorial gene expression in increasing the carotenoid content prior to its application for the generation of biofortified cassava with enhanced carotenoids levels. 2022-01-07 2023-02-09T14:44:36Z 2023-02-09T14:44:36Z Journal Article https://hdl.handle.net/10568/128585 en Open Access application/pdf Jaramillo, A.M.; Sierra, S.; Chavarriaga-Aguirre, P., Castillo, K.D.; Gkanogiannis, A.; Becerra López-Lavalle, L.A.; Arciniegas, J.P.; Sun, T.; Li, L.; Welsch, R.; Boy, E.; Álvarez, D. (2022) Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation. PLoS One 17(1): e0263168 24 p. ISSN: 1932-6203 |
| spellingShingle | provitamins carotenoids protein requirements provitaminas carotenoides requerimientos proteicos vitamin a biofortification health retinol nutrition vitamin deficiencies cassava diet public health Jaramillo, Angélica M. Sierra, Santiago Chavarriaga-Aguirre, Paul Castillo, Diana Katherine Gkanogiannis, Anestis Becerra Lopez-Lavalle, Luis Augusto Arciniegas Vega, Juan Pablo Sun, Tianhu Li, Li Welsch, Ralf Boy, Erick Álvarez, Daniel Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation |
| title | Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation |
| title_full | Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation |
| title_fullStr | Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation |
| title_full_unstemmed | Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation |
| title_short | Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation |
| title_sort | characterization of cassava orange proteins and their capability to increase provitamin a carotenoids accumulation |
| topic | provitamins carotenoids protein requirements provitaminas carotenoides requerimientos proteicos vitamin a biofortification health retinol nutrition vitamin deficiencies cassava diet public health |
| url | https://hdl.handle.net/10568/128585 |
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