Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification
Biofortification of cereal grains offers a lasting solution to combat micronutrient deficiency in developing countries where it poses developmental risks to children. Breeding efforts thus far have been directed toward increasing the grain concentrations of iron (Fe) and zinc (Zn) ions. Phytic acid...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/126372 |
| _version_ | 1855537278401642496 |
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| author | Zhengyu Wen Juliana, Philomin Dhugga, Harshaant S. Pacheco, Mario Martínez, Ulises I. Aguilar, Agustin Ibba, Maria Itria Velu, Govindan Singh, Ravi P. Dhugga, Kanwarpal S. |
| author_browse | Aguilar, Agustin Dhugga, Harshaant S. Dhugga, Kanwarpal S. Ibba, Maria Itria Juliana, Philomin Martínez, Ulises I. Pacheco, Mario Singh, Ravi P. Velu, Govindan Zhengyu Wen |
| author_facet | Zhengyu Wen Juliana, Philomin Dhugga, Harshaant S. Pacheco, Mario Martínez, Ulises I. Aguilar, Agustin Ibba, Maria Itria Velu, Govindan Singh, Ravi P. Dhugga, Kanwarpal S. |
| author_sort | Zhengyu Wen |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Biofortification of cereal grains offers a lasting solution to combat micronutrient deficiency in developing countries where it poses developmental risks to children. Breeding efforts thus far have been directed toward increasing the grain concentrations of iron (Fe) and zinc (Zn) ions. Phytic acid (PA) chelates these metal ions, reducing their bioavailability in the digestive tract. We present a high-throughput assay for quantification of PA and its application in screening a breeding population. After extraction in 96-well megatiter plates, PA content was determined from the phosphate released after treatment with a commercially available phytase enzyme. In a set of 330 breeding lines of wheat grown in the field over 3 years as part of a Harvest Plus breeding program for high grain Fe and Zn, our assay unraveled variation for PA that ranged from 0.90 to 1.72% with a mean of 1.24%. PA content was not associated with grain yield. High yielding lines were further screened for low molar PA/Fe and PA/Zn ratios for increased metal ion bioavailability, demonstrating the utility of our assay. Genome-wide association study revealed 21 genetic associations, six of which were consistent across years. Five of these associations mapped to chromosomes 1A, 2A, 2D, 5A, and 7D. Additivity over four of these haplotypes accounted for an ∼10% reduction in PA. Our study demonstrates it is possible to scale up assays to directly select for low grain PA in forward breeding programs. |
| format | Journal Article |
| id | CGSpace126372 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1263722025-12-08T10:29:22Z Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification Zhengyu Wen Juliana, Philomin Dhugga, Harshaant S. Pacheco, Mario Martínez, Ulises I. Aguilar, Agustin Ibba, Maria Itria Velu, Govindan Singh, Ravi P. Dhugga, Kanwarpal S. biofortification grain nutrition iron zinc phytic acid genomes Biofortification of cereal grains offers a lasting solution to combat micronutrient deficiency in developing countries where it poses developmental risks to children. Breeding efforts thus far have been directed toward increasing the grain concentrations of iron (Fe) and zinc (Zn) ions. Phytic acid (PA) chelates these metal ions, reducing their bioavailability in the digestive tract. We present a high-throughput assay for quantification of PA and its application in screening a breeding population. After extraction in 96-well megatiter plates, PA content was determined from the phosphate released after treatment with a commercially available phytase enzyme. In a set of 330 breeding lines of wheat grown in the field over 3 years as part of a Harvest Plus breeding program for high grain Fe and Zn, our assay unraveled variation for PA that ranged from 0.90 to 1.72% with a mean of 1.24%. PA content was not associated with grain yield. High yielding lines were further screened for low molar PA/Fe and PA/Zn ratios for increased metal ion bioavailability, demonstrating the utility of our assay. Genome-wide association study revealed 21 genetic associations, six of which were consistent across years. Five of these associations mapped to chromosomes 1A, 2A, 2D, 5A, and 7D. Additivity over four of these haplotypes accounted for an ∼10% reduction in PA. Our study demonstrates it is possible to scale up assays to directly select for low grain PA in forward breeding programs. 2022-02-15 2022-12-28T14:59:56Z 2022-12-28T14:59:56Z Journal Article https://hdl.handle.net/10568/126372 en Open Access application/pdf Frontiers Media Wen, Z., Juliana, P., Dhugga, H. S., Pacheco, M., Martínez, U. I., Aguilar, A., Ibba, M. I., Govindan, V., Singh, R. P., & Dhugga, K. S. (2022). Genome-Wide Association Study of Phytic Acid in Wheat Grain Unravels Markers for Improving Biofortification. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.830147 |
| spellingShingle | biofortification grain nutrition iron zinc phytic acid genomes Zhengyu Wen Juliana, Philomin Dhugga, Harshaant S. Pacheco, Mario Martínez, Ulises I. Aguilar, Agustin Ibba, Maria Itria Velu, Govindan Singh, Ravi P. Dhugga, Kanwarpal S. Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification |
| title | Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification |
| title_full | Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification |
| title_fullStr | Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification |
| title_full_unstemmed | Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification |
| title_short | Genome-wide association study of phytic acid in wheat grain unravels markers for improving biofortification |
| title_sort | genome wide association study of phytic acid in wheat grain unravels markers for improving biofortification |
| topic | biofortification grain nutrition iron zinc phytic acid genomes |
| url | https://hdl.handle.net/10568/126372 |
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