Optimization of Palm Kernel Cake Bioconversion with P. ostreatus: An Efficient Lignocellulosic Biomass Value-Adding Process for Ruminant Feed

Optimization of Palm Kernel Cake Bioconversion with P. ostreatus: An Efficient Lignocellulosic Biomass Value-Adding Process for Ruminant Feed

This study aims to optimize the bioconversion of palm kernel cake (PKC) by Pleurotus ostreatus to improve fungal biomass production, lignocellulolytic enzyme expression, and the nutritional value of the substrate as ruminant feed. Three inorganic nitrogen sources (ammonium sulfate, ammonium nitra...

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Bibliographic Details
Main Authors: Ibarra Rondón, Aldo, Durán Sequeda, Dinary Eloisa, Castro Pacheco, Andrea Carolina, Fragoso Castilla, Pedro, Barahona Rosales, Rolando, Mojica Rodríguez, José Edwin
Format: article
Language:Inglés
Published: Multidisciplinary Digital Publishing Institute - MDPI 2025
Subjects:
Genética y mejoramiento animal - L10
Ganado bovino
Genética animal
Hongo
Enzima
Ganadería y especies menores
http://aims.fao.org/aos/agrovoc/c_1391
http://aims.fao.org/aos/agrovoc/c_49986
http://aims.fao.org/aos/agrovoc/c_3145
http://aims.fao.org/aos/agrovoc/c_2603
Online Access:https://www.mdpi.com/2311-5637/11/5/251
http://hdl.handle.net/20.500.12324/41156
https://doi.org/10.3390/fermentation11050251
Description
Summary:This study aims to optimize the bioconversion of palm kernel cake (PKC) by Pleurotus ostreatus to improve fungal biomass production, lignocellulolytic enzyme expression, and the nutritional value of the substrate as ruminant feed. Three inorganic nitrogen sources (ammonium sulfate, ammonium nitrate, and urea) were evaluated for fungal biomass production using a central composite design (CCD) in liquid fermentations. The formulated culture medium (18.72 g/L glucose and 0.39 g/L urea) effectively yielded better fungal biomass production (8 g/L). Based on these results, an extreme vertex design, mixtures with oil palm by-products (PK, hull, and fiber) supplemented with urea, were formulated, finding that PKC stimulated the highest biomass production and laccase enzyme activity in P. ostreatus. The transcriptome of P. ostreatus was obtained, and the chemical composition of the fermented PKC was determined. Transcriptomic analysis revealed the frequency of five key domains with carbohydrate-activated enzyme (CAZy) function: GH3, GH18, CBM1, AA1, and AA5, with activities on lignocellulose. In the fermented PKC, lignin was reduced by 46.9%, and protein was increased by 69.8%. In conclusion, these results show that urea is efficient in the bioconversion of PKC with P. ostreatus as a supplement for ruminants.

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