Optimization of Phage-Based Pathogen Detection During Sample Shipment

Foodborne illnesses are diseases caused by pathogen microorganisms present in the food matrix. Various organizations regulate the laws and guidelines that must be followed to avoid contamination of food with pathogen microorganisms. The presence of pathogen microorganisms is sufficient cause to deny...

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Detalles Bibliográficos
Autor principal:	Cheing G., Roberto S.
Otros Autores:	Márquez, Mayra
Formato:	Tesis
Lenguaje:	Español
Publicado:	Zamorano: Escuela Agrícola Panamericana, 2020. 2020
Materias:	Bacteriophage Bioluminescence Detection E. coli O157:H7
Acceso en línea:	https://bdigital.zamorano.edu/handle/11036/6935

id	ZAMORANO6935
record_format	dspace
spelling	ZAMORANO69352023-03-24T15:03:41Z Optimization of Phage-Based Pathogen Detection During Sample Shipment Cheing G., Roberto S. Márquez, Mayra Applegate, Bruce Bacteriophage Bioluminescence Detection E. coli O157:H7 Foodborne illnesses are diseases caused by pathogen microorganisms present in the food matrix. Various organizations regulate the laws and guidelines that must be followed to avoid contamination of food with pathogen microorganisms. The presence of pathogen microorganisms is sufficient cause to deny the entry of food shipments. That is the reason that rapid detection of pathogen microorganisms is of vital importance. In this study, a bioluminescence detection method for Escherichia coli O157:H7 was evaluated. For this the ΦV10 Lux + Kanamycin resistance was used. A Randomized Complete Block Design (RCB) was used to measure the effect of seven concentrations of E. coli O157:H7, seven concentrations of ΦV10 Lux + Kanamycin resistance, two incubation temperatures, and five enrichment times before the addition of kanamycin. The data were analyzed using a Chi-square test. The sensitivity, specificity, accuracy, positive and negative predictive values of the method were also calculated, obtaining 95, 100, 96, 100 and 36%, respectively. There was no difference in the bioluminescence detection when using bacteria concentrations from 5 Log CFU/mL to 7 Log CFU/mL; phage concentrations from 7 Log PFU/mL to 8 Log PFU/mL (P = 0.9432); 1, 2, 3, 4 or 5 hours enrichment time before adding kanamycin (P = 0.9889) or incubation temperatures of 21 or 37 ˚C (P = 0.9364). 2020-12-01T13:53:50Z 2020-12-01T13:53:50Z 2020 Thesis https://bdigital.zamorano.edu/handle/11036/6935 spa 21 p. Copyright Escuela Agrícola Panamericana, Zamorano, 2020 http://creativecommons.org/licenses/by-nc-nd/3.0/es openAccess application/pdf application/pdf Zamorano Zamorano: Escuela Agrícola Panamericana, 2020.
institution	Universidad Zamorano
collection	Biblioteca Digital Zamorano
language	Español
topic	Bacteriophage Bioluminescence Detection E. coli O157:H7
spellingShingle	Bacteriophage Bioluminescence Detection E. coli O157:H7 Cheing G., Roberto S. Optimization of Phage-Based Pathogen Detection During Sample Shipment
description	Foodborne illnesses are diseases caused by pathogen microorganisms present in the food matrix. Various organizations regulate the laws and guidelines that must be followed to avoid contamination of food with pathogen microorganisms. The presence of pathogen microorganisms is sufficient cause to deny the entry of food shipments. That is the reason that rapid detection of pathogen microorganisms is of vital importance. In this study, a bioluminescence detection method for Escherichia coli O157:H7 was evaluated. For this the ΦV10 Lux + Kanamycin resistance was used. A Randomized Complete Block Design (RCB) was used to measure the effect of seven concentrations of E. coli O157:H7, seven concentrations of ΦV10 Lux + Kanamycin resistance, two incubation temperatures, and five enrichment times before the addition of kanamycin. The data were analyzed using a Chi-square test. The sensitivity, specificity, accuracy, positive and negative predictive values of the method were also calculated, obtaining 95, 100, 96, 100 and 36%, respectively. There was no difference in the bioluminescence detection when using bacteria concentrations from 5 Log CFU/mL to 7 Log CFU/mL; phage concentrations from 7 Log PFU/mL to 8 Log PFU/mL (P = 0.9432); 1, 2, 3, 4 or 5 hours enrichment time before adding kanamycin (P = 0.9889) or incubation temperatures of 21 or 37 ˚C (P = 0.9364).
author2	Márquez, Mayra
author_facet	Márquez, Mayra Cheing G., Roberto S.
format	Tesis
author	Cheing G., Roberto S.
author_sort	Cheing G., Roberto S.
title	Optimization of Phage-Based Pathogen Detection During Sample Shipment
title_short	Optimization of Phage-Based Pathogen Detection During Sample Shipment
title_full	Optimization of Phage-Based Pathogen Detection During Sample Shipment
title_fullStr	Optimization of Phage-Based Pathogen Detection During Sample Shipment
title_full_unstemmed	Optimization of Phage-Based Pathogen Detection During Sample Shipment
title_sort	optimization of phage-based pathogen detection during sample shipment
publisher	Zamorano: Escuela Agrícola Panamericana, 2020.
publishDate	2020
url	https://bdigital.zamorano.edu/handle/11036/6935
work_keys_str_mv	AT cheinggrobertos optimizationofphagebasedpathogendetectionduringsampleshipment
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Optimization of Phage-Based Pathogen Detection During Sample Shipment

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