Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil

Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection for all animals and humans are bites from dogs or bats. The aim of this degree project was to diagnose and determine the source of infection for 11 rabies samples from São Paulo State and Minas Gerais State,...

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Bibliographic Details
Main Author: Torsson, Emeli
Format: H2
Language:Inglés
Swedish
Published: SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2013
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Summary:Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection for all animals and humans are bites from dogs or bats. The aim of this degree project was to diagnose and determine the source of infection for 11 rabies samples from São Paulo State and Minas Gerais State, Brazil. Diagnosis was made through direct immunofluorescence assay, mouse inoculation and RT-PCR. The sources of infection were determined by sequencing 234 nucleotides of the 5’ end of the N-gene and align these with homologous sequences retrieved from GenBank. All seven samples for which satisfactory sequences could be achieved clustered with the sequences related to vampire bat with a bootstrap value of 98%.