Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil
Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection for all animals and humans are bites from dogs or bats. The aim of this degree project was to diagnose and determine the source of infection for 11 rabies samples from São Paulo State and Minas Gerais State,...
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| Formato: | H2 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2013
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| Materias: |
| _version_ | 1855570809828933632 |
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| author | Torsson, Emeli |
| author_browse | Torsson, Emeli |
| author_facet | Torsson, Emeli |
| author_sort | Torsson, Emeli |
| collection | Epsilon Archive for Student Projects |
| description | Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection
for all animals and humans are bites from dogs or bats. The aim of this degree project was to
diagnose and determine the source of infection for 11 rabies samples from São Paulo State
and Minas Gerais State, Brazil. Diagnosis was made through direct immunofluorescence
assay, mouse inoculation and RT-PCR. The sources of infection were determined by
sequencing 234 nucleotides of the 5’ end of the N-gene and align these with homologous
sequences retrieved from GenBank. All seven samples for which satisfactory sequences could
be achieved clustered with the sequences related to vampire bat with a bootstrap value of
98%. |
| format | H2 |
| id | RepoSLU5232 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU52322013-01-29T10:47:06Z Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil Minor Field study - molekylär epidemiologisk studie för rabies i Sao Paulo state and minas Gerais state, Brasilien Torsson, Emeli rabies epidemiology Brazil Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection for all animals and humans are bites from dogs or bats. The aim of this degree project was to diagnose and determine the source of infection for 11 rabies samples from São Paulo State and Minas Gerais State, Brazil. Diagnosis was made through direct immunofluorescence assay, mouse inoculation and RT-PCR. The sources of infection were determined by sequencing 234 nucleotides of the 5’ end of the N-gene and align these with homologous sequences retrieved from GenBank. All seven samples for which satisfactory sequences could be achieved clustered with the sequences related to vampire bat with a bootstrap value of 98%. Rabies är en dödlig zoonos som är spridd över hela världen. De vanligaste smittkällorna för djur och människor är bett från hundar eller fladdermöss. Målet med detta examensarbete var att diagnostisera och fastställa smittkällan för 11 rabiesprover från São Paulo State och Minas Gerais State, Brasilien. Diagnos ställdes via direkt immunofluorescens, musinokulering och RT-PCR. Smittkällan fastställdes via sekvensering av 234 nukleotider av 5´-delen på N-genen vilka jämfördes med homologa sekvenser hämtade från GenBank. Alla sju prover för vilka tillfredställande sekvenser gick att uppnå anhopades, med en sannolikhet på 98 %, inom de sekvenser som relateras till vampyrfladdermusen. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2013 H2 eng swe https://stud.epsilon.slu.se/5232/ |
| spellingShingle | rabies epidemiology Brazil Torsson, Emeli Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil |
| title | Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil |
| title_full | Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil |
| title_fullStr | Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil |
| title_full_unstemmed | Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil |
| title_short | Minor fieled study - molecular epidemiologi of rabies in Sao Paulo state and Minas Gerais state, Brazil |
| title_sort | minor fieled study - molecular epidemiologi of rabies in sao paulo state and minas gerais state, brazil |
| topic | rabies epidemiology Brazil |