Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1
The crystal structure of N10- formyltetrahydrofolate synthetase 1 (FTHFS 1) and N10- formyltetrahydrofolate synthetase 2 (FTHFS 2) from the syntrophic acetate oxidizing bacterium, Tepidanaerobacter acetatoxydans Re1, was solved by x-ray crystallography to a resolution of 2.15 Å, and 2.30 Å resolutio...
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2012
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| Acceso en línea: | https://stud.epsilon.slu.se/5058/ |
| _version_ | 1855570782380359680 |
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| author | Bergdahl, Roland |
| author_browse | Bergdahl, Roland |
| author_facet | Bergdahl, Roland |
| author_sort | Bergdahl, Roland |
| collection | Epsilon Archive for Student Projects |
| description | The crystal structure of N10- formyltetrahydrofolate synthetase 1 (FTHFS 1) and N10- formyltetrahydrofolate synthetase 2 (FTHFS 2) from the syntrophic acetate oxidizing bacterium, Tepidanaerobacter acetatoxydans Re1, was solved by x-ray crystallography to a resolution of 2.15 Å, and 2.30 Å resolution, respectively. The FTHFS 1 crystal structure has four non-crystallographic related protein molecules in the asymmetric unit of the crystal, while FTHFS 2 has two. The pH optimum in the formylation reaction for FTHFS 1 was determined to be 7.5, while FTHFS 2 had a pH optimum of 9, and it was determined that both enzymes had a temperature optimum of 60°C for their formulation reactions. An attempt to perform the deformylation reaction with both enzymes was unsuccessfully carried out. |
| format | Second cycle, A2E |
| id | RepoSLU5058 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2012 |
| publishDateSort | 2012 |
| record_format | eprints |
| spelling | RepoSLU50582013-07-16T23:15:18Z https://stud.epsilon.slu.se/5058/ Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 Bergdahl, Roland Renewable energy resources The crystal structure of N10- formyltetrahydrofolate synthetase 1 (FTHFS 1) and N10- formyltetrahydrofolate synthetase 2 (FTHFS 2) from the syntrophic acetate oxidizing bacterium, Tepidanaerobacter acetatoxydans Re1, was solved by x-ray crystallography to a resolution of 2.15 Å, and 2.30 Å resolution, respectively. The FTHFS 1 crystal structure has four non-crystallographic related protein molecules in the asymmetric unit of the crystal, while FTHFS 2 has two. The pH optimum in the formylation reaction for FTHFS 1 was determined to be 7.5, while FTHFS 2 had a pH optimum of 9, and it was determined that both enzymes had a temperature optimum of 60°C for their formulation reactions. An attempt to perform the deformylation reaction with both enzymes was unsuccessfully carried out. 2012-11-30 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/5058/31/Bergdahl_R_121130.pdf Bergdahl, Roland, 2013. Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1. Second cycle, A2E. Uppsala: (NL, NJ) > Dept. of Molecular Biology (until 131231) <https://stud.epsilon.slu.se/view/divisions/4025.html> urn:nbn:se:slu:epsilon-s-1874 eng |
| spellingShingle | Renewable energy resources Bergdahl, Roland Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 |
| title | Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 |
| title_full | Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 |
| title_fullStr | Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 |
| title_full_unstemmed | Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 |
| title_short | Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 |
| title_sort | characterization, crystallization and two three dimensional structures of the n10- formyltetrahydrofolate synthetase (fthfs) from the syntrophic acetate oxidizing bacterium tepidanaerobacter acetatoxydans re1 |
| topic | Renewable energy resources |
| url | https://stud.epsilon.slu.se/5058/ https://stud.epsilon.slu.se/5058/ |