Troubleshooting the GFP-tagging gene knockout (GGKO) method for the Leptosphaeria maculans effectors AvrLm6 and AvrLm4-7
An attempt was made to GFP-tag the effector proteins of AvrLm6 and AvrLm4-7 using the GFP-tagging gene knockout method (GGKO) developed by Saitoh et al. (2008) in order to determine whether or not they are secreted. Successful pETHG-(target)KO vectors were not generated. The protocol was examined fo...
| Main Author: | |
|---|---|
| Format: | M2 |
| Language: | Inglés Swedish |
| Published: |
SLU/Dept. of Plant Biology and Forest Genetics (until 131231)
2011
|
| Subjects: |
Similar Items: Troubleshooting the GFP-tagging gene knockout (GGKO) method for the Leptosphaeria maculans effectors AvrLm6 and AvrLm4-7
- Estudio epidemiológico del cancro de la base del tallo de colza (Leptosphaeria maculans) en Balcarce, Argentina.
- Identificación de genes de resistencia a Leptosphaeria maculans en genotipos locales de Brassica napus mediante marcadores moleculares
- Logic Model (LM)
- Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants
- Fusion of a bacterial cadherin-like domain and green fluorescent protein as a specific probe to study biofilm matrix formation in Rhizobium spp
- Intermittent Rainfed Rice var. INIA 516 LM1: A Sustainable Alternative for the Huallaga River Basin