Troubleshooting the GFP-tagging gene knockout (GGKO) method for the Leptosphaeria maculans effectors AvrLm6 and AvrLm4-7
An attempt was made to GFP-tag the effector proteins of AvrLm6 and AvrLm4-7 using the GFP-tagging gene knockout method (GGKO) developed by Saitoh et al. (2008) in order to determine whether or not they are secreted. Successful pETHG-(target)KO vectors were not generated. The protocol was examined fo...
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| Formato: | M2 |
| Lenguaje: | Inglés sueco |
| Publicado: |
SLU/Dept. of Plant Biology and Forest Genetics (until 131231)
2011
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| Materias: |
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