In vitro embryo rescue of interspecific hybrids of Lepidium

With the ongoing climate change and growing world population, there is an increasing demand for vegetable oils for industrial and food purposes. Lepidium campestre (field cress) is a wild cruciferous, which is currently under domestication as a new potential high-yielding oil crop for cold climate c...

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Autor principal: Reyes Estevez, Estefany
Formato: Second cycle, A2E
Lenguaje:sueco
Inglés
Publicado: 2021
Materias:
Acceso en línea:https://stud.epsilon.slu.se/17245/
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author Reyes Estevez, Estefany
author_browse Reyes Estevez, Estefany
author_facet Reyes Estevez, Estefany
author_sort Reyes Estevez, Estefany
collection Epsilon Archive for Student Projects
description With the ongoing climate change and growing world population, there is an increasing demand for vegetable oils for industrial and food purposes. Lepidium campestre (field cress) is a wild cruciferous, which is currently under domestication as a new potential high-yielding oil crop for cold climate conditions. Because of problems with early shattering silicles and low oilseed content, there is an initiative to cross the crop with relatives L. draba and L. graminifolium, to achieve a hybrid with shattering resistance and a higher oilseed content. Because of hybrid incompatibility, the hybridization of these species is difficult to achieve with traditional breeding methods. In an attempt to produce a protocol for the successful interspecific crossings of L. campestre x L. draba and L. campestre x L. graminifolium, ovary culture and direct embryo culture, were performed, and various maturity-degrees at the date of harvest were tested. A self-pollinated L. campestre control produced an embryo which developed in vitro, but no hybrid embryos were achieved in the interspecific crosses. It was concluded that experiments with pre-fertilization methods, reciprocal crossing, and more culture media compositions are needed to successfully develop a method for embryo rescuing to obtain interspecific hybrids of L. campestre x L. draba and L. campestre x L. graminifolium.
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institution Swedish University of Agricultural Sciences
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spelling RepoSLU172452021-09-23T01:05:37Z https://stud.epsilon.slu.se/17245/ In vitro embryo rescue of interspecific hybrids of Lepidium Reyes Estevez, Estefany Plant genetics and breeding With the ongoing climate change and growing world population, there is an increasing demand for vegetable oils for industrial and food purposes. Lepidium campestre (field cress) is a wild cruciferous, which is currently under domestication as a new potential high-yielding oil crop for cold climate conditions. Because of problems with early shattering silicles and low oilseed content, there is an initiative to cross the crop with relatives L. draba and L. graminifolium, to achieve a hybrid with shattering resistance and a higher oilseed content. Because of hybrid incompatibility, the hybridization of these species is difficult to achieve with traditional breeding methods. In an attempt to produce a protocol for the successful interspecific crossings of L. campestre x L. draba and L. campestre x L. graminifolium, ovary culture and direct embryo culture, were performed, and various maturity-degrees at the date of harvest were tested. A self-pollinated L. campestre control produced an embryo which developed in vitro, but no hybrid embryos were achieved in the interspecific crosses. It was concluded that experiments with pre-fertilization methods, reciprocal crossing, and more culture media compositions are needed to successfully develop a method for embryo rescuing to obtain interspecific hybrids of L. campestre x L. draba and L. campestre x L. graminifolium. 2021-09-17 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/17245/3/reyes_estevez_estefany_210917.pdf Reyes Estevez, Estefany, 2021. In vitro embryo rescue of interspecific hybrids of Lepidium. Second cycle, A2E. Alnarp: (LTJ, LTV) > Dept. of Plant Breeding (from 130101) <https://stud.epsilon.slu.se/view/divisions/4818.html> urn:nbn:se:slu:epsilon-s-17245 eng
spellingShingle Plant genetics and breeding
Reyes Estevez, Estefany
In vitro embryo rescue of interspecific hybrids of Lepidium
title In vitro embryo rescue of interspecific hybrids of Lepidium
title_full In vitro embryo rescue of interspecific hybrids of Lepidium
title_fullStr In vitro embryo rescue of interspecific hybrids of Lepidium
title_full_unstemmed In vitro embryo rescue of interspecific hybrids of Lepidium
title_short In vitro embryo rescue of interspecific hybrids of Lepidium
title_sort in vitro embryo rescue of interspecific hybrids of lepidium
topic Plant genetics and breeding
url https://stud.epsilon.slu.se/17245/
https://stud.epsilon.slu.se/17245/