The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow
During the oestrous cycle, the porcine cervix undergoes marked macroscopic changes: at oestrus the cervix is firm, and after termination of oestrus it progressively softens. However, little is known about the parallel microscopic changes in the cervix. Therefore, the aim of this study was to examine...
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| Formato: | L3 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231)
2009
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| Materias: |
| _version_ | 1855572038187483136 |
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| author | Edström, Karin |
| author_browse | Edström, Karin |
| author_facet | Edström, Karin |
| author_sort | Edström, Karin |
| collection | Epsilon Archive for Student Projects |
| description | During the oestrous cycle, the porcine cervix undergoes marked macroscopic changes: at oestrus the cervix is firm, and after termination of oestrus it progressively softens. However, little is known about the parallel microscopic changes in the cervix. Therefore, the aim of this study was to examine the histological properties of the epithelium and subepithelial connective tissue of the uterine cervix during oestrus and dioestrus; the morphology of the epithelium and the infiltration of cells of the immune system were described. In addition, the proliferative activities of the epithelium and subepithelial connective tissues were investigated by use of an immunohistochemical method that identifies a nuclear protein (Ki-67) present in proliferating cells only. Six sows, three in oestrus and three in dioestrus, were used for this study.
The cervical epithelium was found to vary between simple columnar, pseudostratified and stratified, with mainly stratified epithelium being found at oestrus and mainly simple columnar at dioestrus. No cyclic variations in the number of immune cells in the epithelium of subepithelial connective tissue could be found. Neither could the proliferative activity be proved to change with different stages of the oestrus cycle. |
| format | L3 |
| id | RepoSLU12300 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) |
| publisherStr | SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) |
| record_format | eprints |
| spelling | RepoSLU123002017-10-31T11:35:33Z The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow Edström, Karin cervix porcine sow morphology immune cells oestrous cycle During the oestrous cycle, the porcine cervix undergoes marked macroscopic changes: at oestrus the cervix is firm, and after termination of oestrus it progressively softens. However, little is known about the parallel microscopic changes in the cervix. Therefore, the aim of this study was to examine the histological properties of the epithelium and subepithelial connective tissue of the uterine cervix during oestrus and dioestrus; the morphology of the epithelium and the infiltration of cells of the immune system were described. In addition, the proliferative activities of the epithelium and subepithelial connective tissues were investigated by use of an immunohistochemical method that identifies a nuclear protein (Ki-67) present in proliferating cells only. Six sows, three in oestrus and three in dioestrus, were used for this study. The cervical epithelium was found to vary between simple columnar, pseudostratified and stratified, with mainly stratified epithelium being found at oestrus and mainly simple columnar at dioestrus. No cyclic variations in the number of immune cells in the epithelium of subepithelial connective tissue could be found. Neither could the proliferative activity be proved to change with different stages of the oestrus cycle. Cervix hos gris genomgår stora makroskopiska förändringar under östralcykeln: under östrus är cervix fast i konsistensen medan den mjuknar då östrus övergår i diöstrus. Det finns dock endast lite kunskap om de samtidiga mikroskopiska förändringarna i cervix. Syftet med denna studie var därför att undersöka de histologiska egenskaperna hos epitel och subepitelial bindväv i den uterina delen av cervix under östrus och diöstrus, med avseende på morfologiska förändringar i epitelet och infiltration av immunceller. Dessutom undersöktes förekomsten av proliferation i epitel och subepitelial bindväv, genom att ett nukleärt protein (Ki- 67) som endast förekommer i prolifererande celler, identifierades med hjälp av en immunohistokemisk metod. I den här studien ingick sex suggor, tre i östrus och tre i diöstrus. Epitelet i cervix varierade mellan enkelt cylinderepitel, flerradigt och flerskiktat epitel. Under östrus fanns huvudsakligen flerskiktat epitel och under diöstrus framför allt enkelt cylinderepitel. Vecken i slemhinnan var tjockare under östrus än diöstrus, vilket tyder på ödem i vävnaden vid östrus. Inga cykliska variation i antalet immunceller i epitelet eller i den subepiteliala bindväven kunde påvisas. Inte heller kunde det bevisas att förekomsten av proliferation varierar mellan östrus och diöstrus. SLU/Dept. of Anatomy, Physiology and Biochemistry (until 231231) 2009 L3 eng swe https://stud.epsilon.slu.se/12300/ |
| spellingShingle | cervix porcine sow morphology immune cells oestrous cycle Edström, Karin The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow |
| title | The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow |
| title_full | The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow |
| title_fullStr | The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow |
| title_full_unstemmed | The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow |
| title_short | The porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow |
| title_sort | porcine cervix : morphological changes and infiltration of immune cells during oestrus and dioestrus in the sow |
| topic | cervix porcine sow morphology immune cells oestrous cycle |