Förekomst av Giardia i symtomfria valpkullar
The flagellate Giardia intestinalis has been known to cause diarrhea in both man and dog. Results from epidemiological studies have indicated that transmission of the parasite from dog to man is plausible even though that route of infection has yet to be shown. Several international studies have...
| Autor principal: | |
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| Formato: | L3 |
| Lenguaje: | sueco Inglés |
| Publicado: |
SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2008
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| Materias: |
| _version_ | 1855571871728140288 |
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| author | Florén, Andreas |
| author_browse | Florén, Andreas |
| author_facet | Florén, Andreas |
| author_sort | Florén, Andreas |
| collection | Epsilon Archive for Student Projects |
| description | The flagellate Giardia intestinalis has been known to cause diarrhea in both man and
dog. Results from epidemiological studies have indicated that transmission of the
parasite from dog to man is plausible even though that route of infection has yet to be
shown. Several international studies have shown that the parasite frequently occurs in
asymptomatic dogs. In Sweden the prevalence of Giardia in healthy dogs is not
known though. In this study, faecal samples were collected at two separate occasions
from 21 litters and 21 bitches showing no symptoms from the gastrointestinal tract.
The samples from each litter were pooled but the samples from the bitches were
analyzed individually. All of the samples were analyzed both by immunofluorescence
(Waterborne Cyst-a-Glo Kit) and an ELISA test (ProSpect Giardia Microplate
Assay).
7 out of 21 (33 %) litters and 2 out of 21 (9.5 %) bitches in this study was harboring
the parasite. 3 of the positive samples were sent to the Swedish Institute for Infectious
Disease Control (SMI) where they were classified based on genotype in order to
evaluate their zoonotic potential. All the analyzed samples belonged to genotypic
group C and D that have never been shown to be able to infect humans.
When comparing the two methods used in this study to detect Giardia it was evident
that ELISA is a more sensitive method than immunofluorescence.
The results indicate that Giardia is a common parasite in the bowel of asymptomatic
Swedish puppies. This is a complicating factor as it makes it rather difficult to assess
positive test results in a clinical environment. |
| format | L3 |
| id | RepoSLU11429 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2008 |
| publishDateSort | 2008 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU114292017-10-05T10:03:01Z Förekomst av Giardia i symtomfria valpkullar Florén, Andreas giardia The flagellate Giardia intestinalis has been known to cause diarrhea in both man and dog. Results from epidemiological studies have indicated that transmission of the parasite from dog to man is plausible even though that route of infection has yet to be shown. Several international studies have shown that the parasite frequently occurs in asymptomatic dogs. In Sweden the prevalence of Giardia in healthy dogs is not known though. In this study, faecal samples were collected at two separate occasions from 21 litters and 21 bitches showing no symptoms from the gastrointestinal tract. The samples from each litter were pooled but the samples from the bitches were analyzed individually. All of the samples were analyzed both by immunofluorescence (Waterborne Cyst-a-Glo Kit) and an ELISA test (ProSpect Giardia Microplate Assay). 7 out of 21 (33 %) litters and 2 out of 21 (9.5 %) bitches in this study was harboring the parasite. 3 of the positive samples were sent to the Swedish Institute for Infectious Disease Control (SMI) where they were classified based on genotype in order to evaluate their zoonotic potential. All the analyzed samples belonged to genotypic group C and D that have never been shown to be able to infect humans. When comparing the two methods used in this study to detect Giardia it was evident that ELISA is a more sensitive method than immunofluorescence. The results indicate that Giardia is a common parasite in the bowel of asymptomatic Swedish puppies. This is a complicating factor as it makes it rather difficult to assess positive test results in a clinical environment. Flagellaten Giardia intestinalis kan orsaka diarréer hos hundar och människor. Epidemiologiska studier tyder på att människor kan smittas av hundar men detta är ännu inte bevisat. Flera internationella studier har visat att parasiten är frekvent förekommande hos symtomfria hundar i många länder. I Sverige är dock prevalensen av Giardia hos symtomfria hundar inte känd. I denna studie samlades avföringsprover vid två tillfällen från 21 valpkullar samt 21 tikar utan gastrointestinala symtom. Samlingsprover togs från valpkullarna och individuella prover från tikarna. Samtliga prover analyserades både med ett kommersiellt immunofluorescenskit (Waterborne Cyst-a-Glo Kit) och en ELISA (ProSpect Giardia Microplate Assay). 7 av 21 (33 %) valpkullar samt 2 av 21 (9,5 %) tikar i studien bar på parasiten. 3 av de positiva proverna skickades till Smittskyddsinstitutet för genotypning med PCR för att kunna bedöma isolatens eventuella zoonotiska potential. Samtliga prover visade sig tillhöra genotypgrupper som aldrig påträffats hos människor. En jämförelse av analysmetoderna visade att ELISA är en känsligare metod för att påvisa Giardia än vad immunofluorescens är. Resultaten tyder på att Giardia är vanligt förekommande hos symtomfria svenska valpar vilket gör det svårt att bedöma ett positivt provsvar i en klinisk situation. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2008 L3 swe eng https://stud.epsilon.slu.se/11429/ |
| spellingShingle | giardia Florén, Andreas Förekomst av Giardia i symtomfria valpkullar |
| title | Förekomst av Giardia i symtomfria valpkullar |
| title_full | Förekomst av Giardia i symtomfria valpkullar |
| title_fullStr | Förekomst av Giardia i symtomfria valpkullar |
| title_full_unstemmed | Förekomst av Giardia i symtomfria valpkullar |
| title_short | Förekomst av Giardia i symtomfria valpkullar |
| title_sort | förekomst av giardia i symtomfria valpkullar |
| topic | giardia |