Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector

Background: Locating the optimal varieties for coffee cultivation is increasingly considered a key condition for sustainable production and marketing. Variety performance varies when it comes to susceptibility to coffee leaf rust and other diseases, adaptation to climate change and high cup quality...

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Autores principales:	Pruvot-Woehl, Solene, Krishnan, Sarada, Solano, William, Schilling, Tim, Toniutti, Lucile, Bertrand, Benoit, Montagnon, Christophe
Formato:	Artículo
Lenguaje:	Inglés
Publicado:	2021
Materias:	COFFEA ARABICA ADN SECTOR CAFETALERO ROYA DEL CAFE CAMBIO CLIMÁTICO ADAPTACIÓN DIVERSIDAD GENETICA AGRICULTORES VULNERABILIDAD RENDIMIENTO
Acceso en línea:	https://repositorio.catie.ac.cr/handle/11554/10314

id	RepoCATIE10314
record_format	dspace
spelling	RepoCATIE103142022-08-05T18:50:36Z Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector Pruvot-Woehl, Solene Krishnan, Sarada Solano, William Schilling, Tim Toniutti, Lucile Bertrand, Benoit Montagnon, Christophe COFFEA ARABICA ADN SECTOR CAFETALERO ROYA DEL CAFE CAMBIO CLIMÁTICO ADAPTACIÓN DIVERSIDAD GENETICA AGRICULTORES VULNERABILIDAD RENDIMIENTO Background: Locating the optimal varieties for coffee cultivation is increasingly considered a key condition for sustainable production and marketing. Variety performance varies when it comes to susceptibility to coffee leaf rust and other diseases, adaptation to climate change and high cup quality for specialty markets. But because of poor organization and the lack of a professional coffee seed sector, most existing coffee farms (and even seed lots and nurseries) do not know which varieties they are using. DNA fingerprinting of coffee planting material will contribute to professionalize the coffee seed sector. Objective: The objective of this paper is i) to check in a large scale the robustness of the existing coffee DNA fingerprinting method based on eight Single Sequence Repeats markers (SRR) and ii) to describe how it can help in moving the needle towards a more professional seed sector. Method: 2533 samples representing all possible genetic background of Arabica varieties were DNA fingerprinted with 8 SRR markers. The genetic diversity was analyzed and the genetic conformity to varietal references was assessed. Results: The DNA fingerprinting method proved to be robust in authenticating varieties and trace back the history of C. arabica breeding and of the movement of C. arabica varieties. The genetic conformity of two important coffee varieties, Marseillesa and Gesha, proved to be 91% and 39% respectively. 2021-02-17T19:38:51Z 2021-02-17T19:38:51Z 2020-01 Artículo doi: 10.1093/jaocint/qsz003 https://repositorio.catie.ac.cr/handle/11554/10314 en Journal of AOAC INTERNATIONAL, (2020), Pages 1–10 info:eu-repo/semantics/openAccess application/pdf
institution	Centro Agronómico Tropical de Investigación y Enseñanza
collection	Repositorio CATIE
language	Inglés
topic	COFFEA ARABICA ADN SECTOR CAFETALERO ROYA DEL CAFE CAMBIO CLIMÁTICO ADAPTACIÓN DIVERSIDAD GENETICA AGRICULTORES VULNERABILIDAD RENDIMIENTO
spellingShingle	COFFEA ARABICA ADN SECTOR CAFETALERO ROYA DEL CAFE CAMBIO CLIMÁTICO ADAPTACIÓN DIVERSIDAD GENETICA AGRICULTORES VULNERABILIDAD RENDIMIENTO Pruvot-Woehl, Solene Krishnan, Sarada Solano, William Schilling, Tim Toniutti, Lucile Bertrand, Benoit Montagnon, Christophe Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector
description	Background: Locating the optimal varieties for coffee cultivation is increasingly considered a key condition for sustainable production and marketing. Variety performance varies when it comes to susceptibility to coffee leaf rust and other diseases, adaptation to climate change and high cup quality for specialty markets. But because of poor organization and the lack of a professional coffee seed sector, most existing coffee farms (and even seed lots and nurseries) do not know which varieties they are using. DNA fingerprinting of coffee planting material will contribute to professionalize the coffee seed sector. Objective: The objective of this paper is i) to check in a large scale the robustness of the existing coffee DNA fingerprinting method based on eight Single Sequence Repeats markers (SRR) and ii) to describe how it can help in moving the needle towards a more professional seed sector. Method: 2533 samples representing all possible genetic background of Arabica varieties were DNA fingerprinted with 8 SRR markers. The genetic diversity was analyzed and the genetic conformity to varietal references was assessed. Results: The DNA fingerprinting method proved to be robust in authenticating varieties and trace back the history of C. arabica breeding and of the movement of C. arabica varieties. The genetic conformity of two important coffee varieties, Marseillesa and Gesha, proved to be 91% and 39% respectively.
format	Artículo
author	Pruvot-Woehl, Solene Krishnan, Sarada Solano, William Schilling, Tim Toniutti, Lucile Bertrand, Benoit Montagnon, Christophe
author_facet	Pruvot-Woehl, Solene Krishnan, Sarada Solano, William Schilling, Tim Toniutti, Lucile Bertrand, Benoit Montagnon, Christophe
author_sort	Pruvot-Woehl, Solene
title	Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector
title_short	Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector
title_full	Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector
title_fullStr	Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector
title_full_unstemmed	Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector
title_sort	authentication of coffea arabica varieties through dna fingerprinting and its significance for the coffee sector
publishDate	2021
url	https://repositorio.catie.ac.cr/handle/11554/10314
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Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector

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