Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content
Twelve cadmium native bacteria previously isolated in soils of cocoa farms located in the western Colombian Andes (Santander), and tolerant to 2500 M CdCl2 (120 mg Cd/L), were chosen in order to test their tolerance and Cd immobilization using liquid culture medium (Nutritive broth) at different co...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Multidisciplinary Digital Publishing Institute - MDPI
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.12324/41370 |
| _version_ | 1855494722733211648 |
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| author | Feria Cáceres, Pedro F. Penagos Velez, Lucas Moreno Herrera, Claudia X. |
| author_browse | Feria Cáceres, Pedro F. Moreno Herrera, Claudia X. Penagos Velez, Lucas |
| author_facet | Feria Cáceres, Pedro F. Penagos Velez, Lucas Moreno Herrera, Claudia X. |
| author_sort | Feria Cáceres, Pedro F. |
| collection | Repositorio AGROSAVIA |
| description | Twelve cadmium native bacteria previously isolated in soils of cocoa farms located in
the western Colombian Andes (Santander), and tolerant to 2500 M CdCl2 (120 mg Cd/L), were
chosen in order to test their tolerance and Cd immobilization using liquid culture medium (Nutritive
broth) at different concentrations of heavy metals. Furthermore, in the greenhouse experiments, the
strains Exiguobacterium sp. (11-4A), Klebsiella variicola sp. (18-4B), and Enterobacter sp. (29-4B) were
applied in combined treatments using CCN51 cacao genotype seeds grown in soil with different
concentrations of Cd. All bacterial strains’ cell morphologies were deformed in TEM pictures, which
also identified six strain interactions with biosorption and four strain capacities for bioaccumulation;
FT-IR suggested that the amide, carbonyl, hydroxyl, ethyl, and phosphate groups on the bacteria
biomass were the main Cd binding sites. In the pot experiments, the concentration of Cd was
distributed throughout the cacao plant, but certain degrees of immobilization of Cd can occur in soil
to prevent an increase in this level in roots with the presence of Klebsiella sp. |
| format | Artículo |
| id | RepoAGROSAVIA41370 |
| institution | Corporación Colombiana de Investigación Agropecuaria |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Multidisciplinary Digital Publishing Institute - MDPI |
| publisherStr | Multidisciplinary Digital Publishing Institute - MDPI |
| record_format | dspace |
| spelling | RepoAGROSAVIA413702025-11-20T14:12:45Z Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content Feria Cáceres, Pedro F. Penagos Velez, Lucas Moreno Herrera, Claudia X. Enfermedades de las plantas - H20 Theobroma cacao Bacteria Cadmio Metal Cacao http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_1178 http://aims.fao.org/aos/agrovoc/c_29036 Twelve cadmium native bacteria previously isolated in soils of cocoa farms located in the western Colombian Andes (Santander), and tolerant to 2500 M CdCl2 (120 mg Cd/L), were chosen in order to test their tolerance and Cd immobilization using liquid culture medium (Nutritive broth) at different concentrations of heavy metals. Furthermore, in the greenhouse experiments, the strains Exiguobacterium sp. (11-4A), Klebsiella variicola sp. (18-4B), and Enterobacter sp. (29-4B) were applied in combined treatments using CCN51 cacao genotype seeds grown in soil with different concentrations of Cd. All bacterial strains’ cell morphologies were deformed in TEM pictures, which also identified six strain interactions with biosorption and four strain capacities for bioaccumulation; FT-IR suggested that the amide, carbonyl, hydroxyl, ethyl, and phosphate groups on the bacteria biomass were the main Cd binding sites. In the pot experiments, the concentration of Cd was distributed throughout the cacao plant, but certain degrees of immobilization of Cd can occur in soil to prevent an increase in this level in roots with the presence of Klebsiella sp. Cacao-Theobroma cacao 2025-11-12T13:24:49Z 2022 2022 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://hdl.handle.net/20.500.12324/41370 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Microbiology Research 2 13 556 573 Zao, M.; Zhang, C.; Zeng, G.; Huang, D.; Xu, P.; Cheng, M. Growth. Growth, metabolism of Phanerochaete chrysosporium and route of lignin degradation in response to cadmium stress in solid-state fermentation. Chemosphere 2015, 138, 560–567. [CrossRef] [PubMed] Chakravarty, R.; Banerjee, P. Mechanism of cadmium binding on the cell wall of an acidophilic bacterium. Bioresour. Technol. 2012, 108, 176–183. [CrossRef] [PubMed] Tossapol, L.; Sooksawat, N. Bioaccumulation and biosorption of Cd2+ and Zn2+ by bacteria isolated from a zinc mine in Thailand. Ecotoxicol. Environ. Saf. 2015, 122, 322–330. [CrossRef] Wong, C.; Cobbett, C. HMA P-type ATPases are the major mechanism for root to shoot Cd translocation in Arabidopsis thaliana. New Phytol. 2009, 181, 71–78. [CrossRef] Shi, Z.; Zhang, Z. Characterization of a high cadmium accumulating soil bacterium, Cupriavidus sp. WS2. Chemosphere 2020, 247, 125834. [CrossRef] Chavez, E.; He, Z. Concentration of cadmium in cacao beans and its relationship with soil cadmium in southern Ecuador. Sci. Total Environ. 2015, 533, 205–214. [CrossRef] [PubMed] Feria-Cáceres, P.; Penagos-Vélez, L. Theobroma cacao L. agricultural soils with natural low and high cadmium (Cd) in Santander (Colombia), contain a persistent shared bacterial composition shaped by multiple soil variables and bacterial isolates highly resistant to Cd concentrations. Curr. Res. Microb. Sci. 2021, 2, 100086. [CrossRef] Huang, F.; Guo, C.-L.; Lu, G.-N.; Yi, X.-Y.; Zhu, L.-D.; Dang, Z. Bioaccumulation characterization of cadmium by growing Bacillus cereus RC-1 and its mechanism. Chemosphere 2014, 109, 134–142. [CrossRef] Li, Y.; Liu, K. Improvement of cadmium phytoremediation by Centella asiatica L. after soil inoculation with cadmium-resistant Enterobacter sp. FM-1. Chemosphere 2018, 202, 280–288. [CrossRef] Verbruggen, N.; Hermans, C. Mechanisms to cope with arsenic or cadmium excess in plants. Curr. Opin. Plant Biol. 2009, 12, 364–372. [CrossRef] Barraza, F.; Schreck, E. Beyond cadmium accumulation: Distribution of other trace elements in soils and cacao beans in Ecuador. Environ. Res. 2021, 192, 110241. [CrossRef] [PubMed Arevalo-Hernandez, C.; Arevalo-Gardini, E. Growth and nutritional responses of wild and domesticated cacao genotypes to soil Cd stress. Sci. Total Environ. 2021, 763, 144021. [CrossRef] [PubMed] Oladipo, O.; Ezeokoli, O. Tolerance and growth kinetics of bacteria isolated from gold and gemstone mining sites in response to heavy metal concentrations. J. Environ. Manag. 2018, 212, 357–366. [CrossRef] [PubMed] application/pdf application/pdf Colombia Multidisciplinary Digital Publishing Institute - MDPI Basilea (Suiza) Microbiology Research; Vol.2, Núm. 13 (2022): Microbiology Research; p.556-573. |
| spellingShingle | Enfermedades de las plantas - H20 Theobroma cacao Bacteria Cadmio Metal Cacao http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_1178 http://aims.fao.org/aos/agrovoc/c_29036 Feria Cáceres, Pedro F. Penagos Velez, Lucas Moreno Herrera, Claudia X. Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content |
| title | Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content |
| title_full | Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content |
| title_fullStr | Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content |
| title_full_unstemmed | Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content |
| title_short | Tolerance and Cadmium (Cd) Immobilization by Native Bacteria Isolated in Cocoa Soils with Increased Metal Content |
| title_sort | tolerance and cadmium cd immobilization by native bacteria isolated in cocoa soils with increased metal content |
| topic | Enfermedades de las plantas - H20 Theobroma cacao Bacteria Cadmio Metal Cacao http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_1178 http://aims.fao.org/aos/agrovoc/c_29036 |
| url | https://hdl.handle.net/20.500.12324/41370 |
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