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Molecular identification of Fusarium species in commercial vanilla and crop wild relatives in Colombia

Vanilla is an economically important crop for low-lying humid tropical regions, but cultivated plants face serious phytosanitary problems. Fusarium wilt is a devastating disease affecting vanilla crops, caused by the fungal pathogens Fusarium oxysporum f. sp. vanillae (Fov) and F. oxysporumf. sp. ra...

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Autores principales: Rodríguez Bastidas, Jayerlin, Manrique Barros, Santiago, Riascos Ortiz, Donald, Mosquera Espinosa, Ana T, S Flanagan, Nicola
Formato: article
Lenguaje:Inglés
Publicado: John Wiley and Sons Inc 2025
Materias:
Cultivo - F01
Fusarium
Vainilla
Cultivo
Comercialización
Hortalizas y plantas aromáticas
http://aims.fao.org/aos/agrovoc/c_3156
http://aims.fao.org/aos/agrovoc/c_15126
http://aims.fao.org/aos/agrovoc/c_1972
http://aims.fao.org/aos/agrovoc/c_21547
Acceso en línea:https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.70038
http://hdl.handle.net/20.500.12324/41186
https://doi.org/10.1111/1758-2229.70038
Sumario:Vanilla is an economically important crop for low-lying humid tropical regions, but cultivated plants face serious phytosanitary problems. Fusarium wilt is a devastating disease affecting vanilla crops, caused by the fungal pathogens Fusarium oxysporum f. sp. vanillae (Fov) and F. oxysporumf. sp. radicis-vanillae (Forv), part of the F. oxysporum species complex(FOSC). We characterized 29 fungal isolates from a vanilla crop and crop wild relatives (CWR) using molecular (EF1-α and ITS-rRNA loci) and morphological traits. Fusarium was the predominant genus, followed by Colleto-trichum and Clonostachys. Four Fusarium species were identified:F. oxysporum (37.9%), Fusarium solani (20.7%), Fusarium pseudocircina-tum (13.8%) and Fusarium concentricum (10.3%). The latter three species were isolated only from CWR and may represent latent pathogens. Fov was isolated from both the crop and CWR, while a Forv-affiliated isolate was also found in a vanilla crop, marking the first report in the neotropical region. TheEF1-α locus provided greater genotype resolution, as well as having refer-ence sequences for Forv. However, the fungal barcode ITS locus is widely applied. We recommend the continued use of both loci for Fusarium diagno-sis in vanilla to facilitate early detection and the development of effective integrated crop management strategies.

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