Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia
A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability of C...
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Semantic Scholar
2025
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| Acceso en línea: | https://www.semanticscholar.org/paper/Isolation-and-typing-of-Clostridium-spp-.-16-S-rRNA-Ortega-Jimenez/87b22bfff9ea0b867b471ff1a9c7819cd7a3eb35?sort=relevance http://hdl.handle.net/20.500.12324/40992 |
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RepoAGROSAVIA40992 |
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dspace |
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Corporación Colombiana de Investigación Agropecuaria |
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Repositorio AGROSAVIA |
| language |
Inglés |
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Genética y mejoramiento animal - L10 Análisis del suelo Variación genética Mortalidad del ganado ADN Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_7198 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_92a58d79 http://aims.fao.org/aos/agrovoc/c_2347 |
| spellingShingle |
Genética y mejoramiento animal - L10 Análisis del suelo Variación genética Mortalidad del ganado ADN Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_7198 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_92a58d79 http://aims.fao.org/aos/agrovoc/c_2347 Ortiz Ortega, Diego Villamil Jiménez, Luis Carlos Rodrigo Martínez, S. Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia |
| description |
A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization
and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by
bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability
of Clostridium genus was analyzed by DNA sequence of a 1500 bp fragment from the 16S rRNA
gene. Twenty four Clostridium isolates were biochemically classified as Clostridium sordellii
(41,7%), Clostridium glycolicum (12,5%), Clostridium hastiforme (12,5%); Clostridium botulinum
(8,3%), Clostridium butyricum (8,3%); Clostridium chauvoei (4,3%),Clostridium limosum (4,3%),
Clostridium septicum (4,3%) and Clostridium tertium (4,3%). The bacteria that showed pathogenic
activity were studied further by 16S rRNA gene sequencing at, which 55.5% was classified as C.
botulinum, where the native strain isolated from areas with outbreaks wasfound in a different
group from Clostridium spp. used by commercial laboratories for vaccine production.
Additionally, the native strain identified here differs from others reported in Gen Bank, indicating
that the native pathogenic Clostridium spp. is genetically different to other Clostridium spp. used
to prepare immunogens affecting vaccine efficiency. Our results indicate that the use of native
strains could improve commercial vaccine preparations, increasing bovine immune response. |
| format |
article |
| author |
Ortiz Ortega, Diego Villamil Jiménez, Luis Carlos Rodrigo Martínez, S. |
| author_facet |
Ortiz Ortega, Diego Villamil Jiménez, Luis Carlos Rodrigo Martínez, S. |
| author_sort |
Ortiz Ortega, Diego |
| title |
Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia |
| title_short |
Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia |
| title_full |
Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia |
| title_fullStr |
Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia |
| title_full_unstemmed |
Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia |
| title_sort |
isolation and typing of clostridium spp.16s rrna from soil samples obtained in areas with sudden mortality history in colombia |
| publisher |
Semantic Scholar |
| publishDate |
2025 |
| url |
https://www.semanticscholar.org/paper/Isolation-and-typing-of-Clostridium-spp-.-16-S-rRNA-Ortega-Jimenez/87b22bfff9ea0b867b471ff1a9c7819cd7a3eb35?sort=relevance http://hdl.handle.net/20.500.12324/40992 |
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AT ortizortegadiego isolationandtypingofclostridiumspp16srrnafromsoilsamplesobtainedinareaswithsuddenmortalityhistoryincolombia AT villamiljimenezluiscarlos isolationandtypingofclostridiumspp16srrnafromsoilsamplesobtainedinareaswithsuddenmortalityhistoryincolombia AT rodrigomartinezs isolationandtypingofclostridiumspp16srrnafromsoilsamplesobtainedinareaswithsuddenmortalityhistoryincolombia |
| _version_ |
1842255961653248000 |
| spelling |
RepoAGROSAVIA409922025-06-14T03:01:25Z Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia Ortiz Ortega, Diego Villamil Jiménez, Luis Carlos Rodrigo Martínez, S. Genética y mejoramiento animal - L10 Análisis del suelo Variación genética Mortalidad del ganado ADN Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_7198 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_92a58d79 http://aims.fao.org/aos/agrovoc/c_2347 A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability of Clostridium genus was analyzed by DNA sequence of a 1500 bp fragment from the 16S rRNA gene. Twenty four Clostridium isolates were biochemically classified as Clostridium sordellii (41,7%), Clostridium glycolicum (12,5%), Clostridium hastiforme (12,5%); Clostridium botulinum (8,3%), Clostridium butyricum (8,3%); Clostridium chauvoei (4,3%),Clostridium limosum (4,3%), Clostridium septicum (4,3%) and Clostridium tertium (4,3%). The bacteria that showed pathogenic activity were studied further by 16S rRNA gene sequencing at, which 55.5% was classified as C. botulinum, where the native strain isolated from areas with outbreaks wasfound in a different group from Clostridium spp. used by commercial laboratories for vaccine production. Additionally, the native strain identified here differs from others reported in Gen Bank, indicating that the native pathogenic Clostridium spp. is genetically different to other Clostridium spp. used to prepare immunogens affecting vaccine efficiency. Our results indicate that the use of native strains could improve commercial vaccine preparations, increasing bovine immune response. Ganadería bovina 2025-06-13T19:13:08Z 2025-06-13T19:13:08Z 2012-04 2012 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.semanticscholar.org/paper/Isolation-and-typing-of-Clostridium-spp-.-16-S-rRNA-Ortega-Jimenez/87b22bfff9ea0b867b471ff1a9c7819cd7a3eb35?sort=relevance 2315-5018 http://hdl.handle.net/20.500.12324/40992 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Global Advanced Research Journal of Microbiology 1 3 33 40 Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez- Garayzabal J, Garcia P, Cai J, Hippe H, Farrow JAE (1994). The Phylogeny of the Genus Clostridium: Proposal of Five New Genera and Eleven New Species Combinations. 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Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf C.I Tibaitatá Colombia Semantic Scholar Global Advanced Research Journal of Microbiology; Vol. 1, Núm. 3 (2012): Global Advanced Research Journal of Microbiology (Abri.);p. 33 - 40. |