Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia
A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability of C...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Semantic Scholar
2025
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| Materias: | |
| Acceso en línea: | https://www.semanticscholar.org/paper/Isolation-and-typing-of-Clostridium-spp-.-16-S-rRNA-Ortega-Jimenez/87b22bfff9ea0b867b471ff1a9c7819cd7a3eb35?sort=relevance http://hdl.handle.net/20.500.12324/40992 |
| Sumario: | A Longitudinal epidemiologic study was developed for the isolation, biochemical characterization
and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by
bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability
of Clostridium genus was analyzed by DNA sequence of a 1500 bp fragment from the 16S rRNA
gene. Twenty four Clostridium isolates were biochemically classified as Clostridium sordellii
(41,7%), Clostridium glycolicum (12,5%), Clostridium hastiforme (12,5%); Clostridium botulinum
(8,3%), Clostridium butyricum (8,3%); Clostridium chauvoei (4,3%),Clostridium limosum (4,3%),
Clostridium septicum (4,3%) and Clostridium tertium (4,3%). The bacteria that showed pathogenic
activity were studied further by 16S rRNA gene sequencing at, which 55.5% was classified as C.
botulinum, where the native strain isolated from areas with outbreaks wasfound in a different
group from Clostridium spp. used by commercial laboratories for vaccine production.
Additionally, the native strain identified here differs from others reported in Gen Bank, indicating
that the native pathogenic Clostridium spp. is genetically different to other Clostridium spp. used
to prepare immunogens affecting vaccine efficiency. Our results indicate that the use of native
strains could improve commercial vaccine preparations, increasing bovine immune response. |
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