Masked genotype 6 of bovine leukemia virus is found in colombian cattle
Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV)....
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Research Square
2025
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| Online Access: | https://www.researchsquare.com/article/rs-2065/v1 http://hdl.handle.net/20.500.12324/40690 https://doi.org/10.21203/rs.2.11155/v1 |
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RepoAGROSAVIA40690 |
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Corporación Colombiana de Investigación Agropecuaria |
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Repositorio AGROSAVIA |
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Inglés |
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Enfermedades de los animales - L73 Ganado bovino Enfermedades de los animales Leucemia Virus Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_4297 http://aims.fao.org/aos/agrovoc/c_8262 |
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Enfermedades de los animales - L73 Ganado bovino Enfermedades de los animales Leucemia Virus Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_4297 http://aims.fao.org/aos/agrovoc/c_8262 Corredor Figueroa, Adriana Patricia Olaya Galán, Nury Nathalia Salas, Sandra Patricia Quintero, Juan Sebastián Fajardo, Álvaro Soñora, Martín Moreno, Pilar Cristina, Juan Sánchez, Alfredo Tobón, Julio Ortiz, Diego Gutierrez, María Fernanda Masked genotype 6 of bovine leukemia virus is found in colombian cattle |
| description |
Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV). Ten BLV genotypes have been reported based on analysis of complete or partial envelope gene sequences, of which genotype 1 is the most prevalent. However, the genetic variability of BLV variants circulating in Colombia remains unexplored. Therefore, in the present study, we analyzed blood samples collected from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses.
Results
A total of 62% of the cows present at 92% of the farms were BLV-positive. Genotype 1 was exclusively detected by env and gag gene segments when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes.
Conclusions
This report is the first molecular and epidemiological study of BLV conducted in Colombia and contributes to the global epidemiology of the virus; it also reinforces the great impact of BLV on the country’s livestock and thus is a useful resource for farmers and government entities. |
| format |
article |
| author |
Corredor Figueroa, Adriana Patricia Olaya Galán, Nury Nathalia Salas, Sandra Patricia Quintero, Juan Sebastián Fajardo, Álvaro Soñora, Martín Moreno, Pilar Cristina, Juan Sánchez, Alfredo Tobón, Julio Ortiz, Diego Gutierrez, María Fernanda |
| author_facet |
Corredor Figueroa, Adriana Patricia Olaya Galán, Nury Nathalia Salas, Sandra Patricia Quintero, Juan Sebastián Fajardo, Álvaro Soñora, Martín Moreno, Pilar Cristina, Juan Sánchez, Alfredo Tobón, Julio Ortiz, Diego Gutierrez, María Fernanda |
| author_sort |
Corredor Figueroa, Adriana Patricia |
| title |
Masked genotype 6 of bovine leukemia virus is found in colombian cattle |
| title_short |
Masked genotype 6 of bovine leukemia virus is found in colombian cattle |
| title_full |
Masked genotype 6 of bovine leukemia virus is found in colombian cattle |
| title_fullStr |
Masked genotype 6 of bovine leukemia virus is found in colombian cattle |
| title_full_unstemmed |
Masked genotype 6 of bovine leukemia virus is found in colombian cattle |
| title_sort |
masked genotype 6 of bovine leukemia virus is found in colombian cattle |
| publisher |
Research Square |
| publishDate |
2025 |
| url |
https://www.researchsquare.com/article/rs-2065/v1 http://hdl.handle.net/20.500.12324/40690 https://doi.org/10.21203/rs.2.11155/v1 |
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1842255583651037184 |
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RepoAGROSAVIA406902025-02-14T03:00:26Z Masked genotype 6 of bovine leukemia virus is found in colombian cattle MASKED GENOTYPE 6 OF BOVINE LEUKEMIA VIRUS IS FOUND IN COLOMBIAN CATTLE Corredor Figueroa, Adriana Patricia Olaya Galán, Nury Nathalia Salas, Sandra Patricia Quintero, Juan Sebastián Fajardo, Álvaro Soñora, Martín Moreno, Pilar Cristina, Juan Sánchez, Alfredo Tobón, Julio Ortiz, Diego Gutierrez, María Fernanda Enfermedades de los animales - L73 Ganado bovino Enfermedades de los animales Leucemia Virus Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_4297 http://aims.fao.org/aos/agrovoc/c_8262 Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV). Ten BLV genotypes have been reported based on analysis of complete or partial envelope gene sequences, of which genotype 1 is the most prevalent. However, the genetic variability of BLV variants circulating in Colombia remains unexplored. Therefore, in the present study, we analyzed blood samples collected from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. Results A total of 62% of the cows present at 92% of the farms were BLV-positive. Genotype 1 was exclusively detected by env and gag gene segments when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. Conclusions This report is the first molecular and epidemiological study of BLV conducted in Colombia and contributes to the global epidemiology of the virus; it also reinforces the great impact of BLV on the country’s livestock and thus is a useful resource for farmers and government entities. Ministerio de Ciencia Tecnología e Innovación - Colciencias Ganadería bovina 2025-02-13T16:17:11Z 2025-02-13T16:17:11Z 2019-07 2019 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.researchsquare.com/article/rs-2065/v1 2693-5015 http://hdl.handle.net/20.500.12324/40690 https://doi.org/10.21203/rs.2.11155/v1 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Research article 1 3 1 20 Ortiz D, Sanchez A, Tobón J, Chaparro y, Gutierrez M: Leucosis Bovina Enzootica, Enzotic bovine leukosis, epidemiological indicators for establishing control and prevention measures. Vol., pp. , May Journal of Veterinary Medicine and Animal Health 2016, 8(5):35-43. 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