Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition
Hyperhydricity can affect the development of plant morphology. A better understanding of the anatomical and physiological changes of hyperhydric plants is needed to predict and control the occurrence of hyperhydryc conditions. The aim of this study is to demonstrate the role of oxidative stress in...
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Research Square
2024
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| Online Access: | https://www.researchsquare.com/article/rs-1886347 http://hdl.handle.net/20.500.12324/40151 https://doi.org/10.21203/rs.3.rs-1886347/v1 |
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Corporación Colombiana de Investigación Agropecuaria |
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Repositorio AGROSAVIA |
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Inglés |
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Genética vegetal y fitomejoramiento - F30 Bambusa vulgaris Morfología Brote In vitro Transversal http://aims.fao.org/aos/agrovoc/c_805 http://aims.fao.org/aos/agrovoc/c_49903 http://aims.fao.org/aos/agrovoc/c_14261 http://aims.fao.org/aos/agrovoc/c_37563 |
| spellingShingle |
Genética vegetal y fitomejoramiento - F30 Bambusa vulgaris Morfología Brote In vitro Transversal http://aims.fao.org/aos/agrovoc/c_805 http://aims.fao.org/aos/agrovoc/c_49903 http://aims.fao.org/aos/agrovoc/c_14261 http://aims.fao.org/aos/agrovoc/c_37563 Garcia, Yudith Yanet Barrera, Gloria Patricia Freire, Marisol Barbón, Raúl Torres, Sinesio Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition |
| description |
Hyperhydricity can affect the development of plant morphology. A better understanding of the anatomical
and physiological changes of hyperhydric plants is needed to predict and control the occurrence of
hyperhydryc conditions. The aim of this study is to demonstrate the role of oxidative stress in
hyperhydricity. To this end, the anatomical, physiological and biochemical responses to oxidative stress
in shoots of Bambusa vulgaris Schrad. ex Wendl were compared during the in vitro-ex vitro transition. For
this propose, we used shoots grown in two different culture systems: liquid static culture medium and
temporary immersion system. Our results showed that hyperhydricity was associated with oxidative
stress in the shoots. In hyperhydric shoots chlorophyll content decreased when cultured in liquid static
culture medium. Moreover, hydrogen peroxide content and malondialdehyde, as well as the activities of
catalase and enzymes of ascorbate-glutathione cycle (ascorbate peroxidase, monodehydroascorbate
reductase and dehydroascorbate reductase) were increased in these shoots. On the other hand, scanning
electron microscopy showed that the leaves of hyperhydric shoots exhibited anatomical changes in the
stomata of the plants, whereas the leaves of normal shoots showed normal structural development.
Finally, normal shoots showed high survival rate and allowed better adaptation of the plantlets in the
greenhouse. |
| format |
article |
| author |
Garcia, Yudith Yanet Barrera, Gloria Patricia Freire, Marisol Barbón, Raúl Torres, Sinesio |
| author_facet |
Garcia, Yudith Yanet Barrera, Gloria Patricia Freire, Marisol Barbón, Raúl Torres, Sinesio |
| author_sort |
Garcia, Yudith Yanet |
| title |
Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition |
| title_short |
Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition |
| title_full |
Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition |
| title_fullStr |
Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition |
| title_full_unstemmed |
Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition |
| title_sort |
anatomical and biochemical responses to oxidative stress in shoots of bambusa vulgaris schrad. ex wendl during the in vitro-ex vitro transition |
| publisher |
Research Square |
| publishDate |
2024 |
| url |
https://www.researchsquare.com/article/rs-1886347 http://hdl.handle.net/20.500.12324/40151 https://doi.org/10.21203/rs.3.rs-1886347/v1 |
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| spelling |
RepoAGROSAVIA401512024-09-21T03:02:54Z Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition Garcia, Yudith Yanet Barrera, Gloria Patricia Freire, Marisol Barbón, Raúl Torres, Sinesio Genética vegetal y fitomejoramiento - F30 Bambusa vulgaris Morfología Brote In vitro Transversal http://aims.fao.org/aos/agrovoc/c_805 http://aims.fao.org/aos/agrovoc/c_49903 http://aims.fao.org/aos/agrovoc/c_14261 http://aims.fao.org/aos/agrovoc/c_37563 Hyperhydricity can affect the development of plant morphology. A better understanding of the anatomical and physiological changes of hyperhydric plants is needed to predict and control the occurrence of hyperhydryc conditions. The aim of this study is to demonstrate the role of oxidative stress in hyperhydricity. To this end, the anatomical, physiological and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl were compared during the in vitro-ex vitro transition. For this propose, we used shoots grown in two different culture systems: liquid static culture medium and temporary immersion system. Our results showed that hyperhydricity was associated with oxidative stress in the shoots. In hyperhydric shoots chlorophyll content decreased when cultured in liquid static culture medium. Moreover, hydrogen peroxide content and malondialdehyde, as well as the activities of catalase and enzymes of ascorbate-glutathione cycle (ascorbate peroxidase, monodehydroascorbate reductase and dehydroascorbate reductase) were increased in these shoots. On the other hand, scanning electron microscopy showed that the leaves of hyperhydric shoots exhibited anatomical changes in the stomata of the plants, whereas the leaves of normal shoots showed normal structural development. Finally, normal shoots showed high survival rate and allowed better adaptation of the plantlets in the greenhouse. Educational Network in Agrobiodiversity - EDUNABIO 2024-09-20T15:04:09Z 2024-09-20T15:04:09Z 2022-08-01 2022 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.researchsquare.com/article/rs-1886347 2693-501 http://hdl.handle.net/20.500.12324/40151 https://doi.org/10.21203/rs.3.rs-1886347/v1 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Research Square 1 1 1 16 Aebi H (1984) Catalase in vitro. In Methods in Enzymology. 105: 121–126. https://doi.org/10.1016/S0076-6879(84)05016-3 Apóstolo NM, Llorente BE (2000) Anatomy of normal and hyperhydric leaves and shoots of in vitro grown Simmondsia chinesis (Link) Schn. 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