The role of division stochasticity on the robustness of bacterial size dynamics

Variables of bacterial division such as size at birth, growth rate, division time, and the position of the septal ring, all vary from cell to cell. Currently, it is unknown how these random fluctuations can combine to produce a robust mechanism of homeostasis. To address this question, we studied th...

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Main Authors:	Nieto, César, Arias Castro, Juan Carlos, Sánchez, Carlos, Vargas García, César, Singh, Abhyudai, Pedraza, Juan Manuel
Format:	article
Language:	Inglés
Published:	Cold Sprimg Harbor Laboratory (CSH) 2024
Subjects:	Investigación agropecuaria - A50 Bacteria Homeostasis Celdas de parición Ciclo celular Transversal http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_2812 http://aims.fao.org/aos/agrovoc/c_37218
Online Access:	https://www.biorxiv.org/content/10.1101/2022.07.27.501776v1.article-info http://hdl.handle.net/20.500.12324/39917 https://doi.org/10.1101/2022.07.27.501776

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record_format	dspace
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Investigación agropecuaria - A50 Bacteria Homeostasis Celdas de parición Ciclo celular Transversal http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_2812 http://aims.fao.org/aos/agrovoc/c_37218
spellingShingle	Investigación agropecuaria - A50 Bacteria Homeostasis Celdas de parición Ciclo celular Transversal http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_2812 http://aims.fao.org/aos/agrovoc/c_37218 Nieto, César Arias Castro, Juan Carlos Sánchez, Carlos Vargas García, César Singh, Abhyudai Pedraza, Juan Manuel The role of division stochasticity on the robustness of bacterial size dynamics
description	Variables of bacterial division such as size at birth, growth rate, division time, and the position of the septal ring, all vary from cell to cell. Currently, it is unknown how these random fluctuations can combine to produce a robust mechanism of homeostasis. To address this question, we studied the dynamics of the cell division process from both experimental and theoretical perspectives. Our model predicts robustness in division times as sustained oscillations in metrics of the cell size distribution, such as the mean, variability, and the cell size autocorrelation function. These oscillations do not get damped, even considering stochasticity in division timing and the cell size at the beginning of the experiment. Damping appears just after inducing stochasticity in either the septum position or the growth rate. We compare the predictions of the full model with the size dynamics of E. coli bacteria growing in minimal media using either glucose or glycerol as carbon sources. We observe that growth in poorer media increases the noise in both partitioning position and growth rate. This additional noise results in oscillations with more damping. Although intracellular noise is known as a source of phenotypic variation, our results show that it can play a similar but subtler role in maintaining population-level homeostasis by causing rapid desynchronization of cell cycles..
format	article
author	Nieto, César Arias Castro, Juan Carlos Sánchez, Carlos Vargas García, César Singh, Abhyudai Pedraza, Juan Manuel
author_facet	Nieto, César Arias Castro, Juan Carlos Sánchez, Carlos Vargas García, César Singh, Abhyudai Pedraza, Juan Manuel
author_sort	Nieto, César
title	The role of division stochasticity on the robustness of bacterial size dynamics
title_short	The role of division stochasticity on the robustness of bacterial size dynamics
title_full	The role of division stochasticity on the robustness of bacterial size dynamics
title_fullStr	The role of division stochasticity on the robustness of bacterial size dynamics
title_full_unstemmed	The role of division stochasticity on the robustness of bacterial size dynamics
title_sort	role of division stochasticity on the robustness of bacterial size dynamics
publisher	Cold Sprimg Harbor Laboratory (CSH)
publishDate	2024
url	https://www.biorxiv.org/content/10.1101/2022.07.27.501776v1.article-info http://hdl.handle.net/20.500.12324/39917 https://doi.org/10.1101/2022.07.27.501776
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spelling	RepoAGROSAVIA399172024-08-24T03:02:27Z The role of division stochasticity on the robustness of bacterial size dynamics The role of division stochasticity on the robustness of bacterial size dynamics Nieto, César Arias Castro, Juan Carlos Sánchez, Carlos Vargas García, César Singh, Abhyudai Pedraza, Juan Manuel Investigación agropecuaria - A50 Bacteria Homeostasis Celdas de parición Ciclo celular Transversal http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_2812 http://aims.fao.org/aos/agrovoc/c_37218 Variables of bacterial division such as size at birth, growth rate, division time, and the position of the septal ring, all vary from cell to cell. Currently, it is unknown how these random fluctuations can combine to produce a robust mechanism of homeostasis. To address this question, we studied the dynamics of the cell division process from both experimental and theoretical perspectives. Our model predicts robustness in division times as sustained oscillations in metrics of the cell size distribution, such as the mean, variability, and the cell size autocorrelation function. These oscillations do not get damped, even considering stochasticity in division timing and the cell size at the beginning of the experiment. Damping appears just after inducing stochasticity in either the septum position or the growth rate. We compare the predictions of the full model with the size dynamics of E. coli bacteria growing in minimal media using either glucose or glycerol as carbon sources. We observe that growth in poorer media increases the noise in both partitioning position and growth rate. This additional noise results in oscillations with more damping. Although intracellular noise is known as a source of phenotypic variation, our results show that it can play a similar but subtler role in maintaining population-level homeostasis by causing rapid desynchronization of cell cycles.. 2024-08-23T15:59:21Z 2024-08-23T15:59:21Z 2022-07 2022 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.biorxiv.org/content/10.1101/2022.07.27.501776v1.article-info http://hdl.handle.net/20.500.12324/39917 https://doi.org/10.1101/2022.07.27.501776 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng BioRxiv 1 1 11 Y.-M. Zhang and C. O. Rock, “Membrane lipid homeostasis in bacteria,” Nature Reviews Microbiology, vol. 6, no. 3, pp. 222–233, 2008. N. 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The role of division stochasticity on the robustness of bacterial size dynamics

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