Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil

The aim of this study was isolation and identification of rhizospheric fungi in association with cacti [Opuntia cochenillifera (Linnaeus) Miller] in an area under salinization and desertification, as well as to model their chemical and mineralogical attributes. The experimental area was located in t...

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Autores principales:	João Manoel, da Silva, Araujo Dalbon, Viviane, la Rosa Massahud, Regla Toujaguez, Molina Acevedo, Juan Pablo, Machado de Medeiros Ferro, Mayra, Carvalho dos Santos, Tania Marta, Simões do Amaral Alves, Elizabeth, Andrade Lima, Gaus Silvestre
Formato:	article
Lenguaje:	Inglés
Publicado:	International Journal Of Agriculture & Biology 2024
Materias:	Cultivo - F01 Aspergillus Hongos Propiedades fisicoquímicas Cultivo Transversal http://aims.fao.org/aos/agrovoc/c_672 http://aims.fao.org/aos/agrovoc/c_3145 http://aims.fao.org/aos/agrovoc/c_1521
Acceso en línea:	http://hdl.handle.net/20.500.12324/39375

id	RepoAGROSAVIA39375
record_format	dspace
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Cultivo - F01 Aspergillus Hongos Propiedades fisicoquímicas Cultivo Transversal http://aims.fao.org/aos/agrovoc/c_672 http://aims.fao.org/aos/agrovoc/c_3145 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_3145
spellingShingle	Cultivo - F01 Aspergillus Hongos Propiedades fisicoquímicas Cultivo Transversal http://aims.fao.org/aos/agrovoc/c_672 http://aims.fao.org/aos/agrovoc/c_3145 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_3145 João Manoel, da Silva Araujo Dalbon, Viviane la Rosa Massahud, Regla Toujaguez Molina Acevedo, Juan Pablo Machado de Medeiros Ferro, Mayra Carvalho dos Santos, Tania Marta Simões do Amaral Alves, Elizabeth Andrade Lima, Gaus Silvestre Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil
description	The aim of this study was isolation and identification of rhizospheric fungi in association with cacti [Opuntia cochenillifera (Linnaeus) Miller] in an area under salinization and desertification, as well as to model their chemical and mineralogical attributes. The experimental area was located in the municipality of Ouro Branco-AL, Brazil. To determine the microbial population, rhizospheric soil was collected from a depth of 0–20 cm. The dilution was carried out to fractions 10-3, 10-4 and 10-5 and inoculated in Petri dishes containing selective culture media. A count of colony forming units (CFU), and morphological identification was performed. The isolates were subjected to DNA extraction, followed by amplification through PCR. The sequences were analyzed with Staden Package and MEGA softwares, followed by the BLASTn search in GenBank. Through the phylogenetic analysis of the rDNA ITS, the species belonging to Coprinellus, Paecilomyces, Aspergillus, Penicillium, and Neurospora were identified. These genera are in the literature for their metabolic richness, being applicable to biotechnological processes for growth promotion in plants, as well as other processes of industrial interest. © 2022 Friends Science Publishers
format	article
author	João Manoel, da Silva Araujo Dalbon, Viviane la Rosa Massahud, Regla Toujaguez Molina Acevedo, Juan Pablo Machado de Medeiros Ferro, Mayra Carvalho dos Santos, Tania Marta Simões do Amaral Alves, Elizabeth Andrade Lima, Gaus Silvestre
author_facet	João Manoel, da Silva Araujo Dalbon, Viviane la Rosa Massahud, Regla Toujaguez Molina Acevedo, Juan Pablo Machado de Medeiros Ferro, Mayra Carvalho dos Santos, Tania Marta Simões do Amaral Alves, Elizabeth Andrade Lima, Gaus Silvestre
author_sort	João Manoel, da Silva
title	Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil
title_short	Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil
title_full	Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil
title_fullStr	Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil
title_full_unstemmed	Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil
title_sort	isolation and molecular identification of rhizospheric fungi associated with opuntia cochenillifera from semiarid in alagoas, brazil
publisher	International Journal Of Agriculture & Biology
publishDate	2024
url	http://hdl.handle.net/20.500.12324/39375
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spelling	RepoAGROSAVIA393752024-06-20T18:40:37Z Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil João Manoel, da Silva Araujo Dalbon, Viviane la Rosa Massahud, Regla Toujaguez Molina Acevedo, Juan Pablo Machado de Medeiros Ferro, Mayra Carvalho dos Santos, Tania Marta Simões do Amaral Alves, Elizabeth Andrade Lima, Gaus Silvestre Cultivo - F01 Aspergillus Hongos Propiedades fisicoquímicas Cultivo Transversal http://aims.fao.org/aos/agrovoc/c_672 http://aims.fao.org/aos/agrovoc/c_3145 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_3145 The aim of this study was isolation and identification of rhizospheric fungi in association with cacti [Opuntia cochenillifera (Linnaeus) Miller] in an area under salinization and desertification, as well as to model their chemical and mineralogical attributes. The experimental area was located in the municipality of Ouro Branco-AL, Brazil. To determine the microbial population, rhizospheric soil was collected from a depth of 0–20 cm. The dilution was carried out to fractions 10-3, 10-4 and 10-5 and inoculated in Petri dishes containing selective culture media. A count of colony forming units (CFU), and morphological identification was performed. The isolates were subjected to DNA extraction, followed by amplification through PCR. The sequences were analyzed with Staden Package and MEGA softwares, followed by the BLASTn search in GenBank. Through the phylogenetic analysis of the rDNA ITS, the species belonging to Coprinellus, Paecilomyces, Aspergillus, Penicillium, and Neurospora were identified. These genera are in the literature for their metabolic richness, being applicable to biotechnological processes for growth promotion in plants, as well as other processes of industrial interest. © 2022 Friends Science Publishers 2024-05-22T15:57:29Z 2024-05-22T15:57:29Z 2022-06-15 2022 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 1814-9596 http://hdl.handle.net/20.500.12324/39375 10.17957/IJAB/15.1937 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng International Journal of Agriculture and Biology 27 6 371 380 Adl SM (2016). Rhizosphere, food security, and climate change: A critical role for plant-soil research. Rhizosphere 1:1–3 Ahkami AH, RA White III, PP Handakumbura, C Jansson (2017) . 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Isolation and Molecular Identification of Rhizospheric Fungi Associated with Opuntia cochenillifera from Semiarid in Alagoas, Brazil

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