Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum
Vascular wilt caused by the fungus Fusarium oxysporum is considered the main constraint of cape gooseberry, Physalis peruviana, production in Colombia. P. peruviana and P. floridana genotypes with differential resistance responses against F. oxysporum have been identified previously. In the present...
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Corporación colombiana de investigación agropecuaria - AGROSAVIA
2019
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| Acceso en línea: | http://revistacta.agrosavia.co/index.php/revista/article/view/396 http://hdl.handle.net/20.500.12324/35186 |
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RepoAGROSAVIA351862024-06-18T21:03:32Z Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum Citogenética de genotipos de uchuva, Physalis peruviana L., y Physalis floridana Rydb., con respuesta diferencial a Fusarium oxysporum Liberato Guío, Sara Alejandra Sánchez Betancourt, Érika Patricia Arguelles Cárdenas, Jorge González Almario, Carolina Núñez Zarante, Víctor Manuel Frutales Vascular wilt caused by the fungus Fusarium oxysporum is considered the main constraint of cape gooseberry, Physalis peruviana, production in Colombia. P. peruviana and P. floridana genotypes with differential resistance responses against F. oxysporum have been identified previously. In the present study, the genotypes were evaluated in order to complement the knowledge of cytogenetics diversity in Physalis and to design hybridization strategies to support breeding of cape gooseberry crop. The chromosome number in mitotic dividing cells from root-tips of tissue culture plantlets was determined, from which the average mitotic hour was estimated at 12:00 hours for P. peruviana and 10:00 for P. floridana. Chromosomic complements of 2n = 4x = 48 and 2n = 2x = 24 were found for each one of the two species. Additionally, flow cytometry analyses detected variation within P. peruviana with a nuclear DNA content of 2.33 pg for the 2n = 24 genotype and variations ranged from 5.77 to 8.12 pg for 2n = 48 genotypes. In P. floridana DNA content was 2.29 pg in the 2n = 24 genotype and 4.03 pg in the 2n = 48 genotype. There was a significant effect (α = 0.01) of the number of chromosomes on nuclear DNA content for the two species El marchitamiento vascular ocasionado por el hongo Fusarium oxysporum se considera el principal limitante en la producción de uchuva (Physalis peruviana) en Colombia. Se han identificado genotipos de P. peruviana y la especie relacionada P. floridana con diferentes niveles de reacción a F. oxysporum. En el presente estudio, se evaluaron los genotipos con el fin de complementar el conocimiento de la diversidad citogenética en Physalis y el diseño de estrategias de hibridación como apoyo al mejoramiento del cultivo de la uchuva. Se determinó el número de cromosomas en células en división mitótica de ápices radicales de plantas cultivadas in vitro, para lo cual se estimó como hora mitótica promedio las 12:00 para P. peruviana, y las 10:00 para P. floridana. Se encontraron dotaciones cromosómicas de 2n = 4x = 48 y 2n = 2x = 24 para cada una de las dos especies. Adicionalmente, se realizó un análisis por citometría de flujo, en el cual se detectó variación en el contenido de ADN nuclear en P. peruviana con 2,33 pg para el genotipo 2n = 24 y entre 5,77 y 8,12 pg para los genotipos 2n = 48. En P. floridana el contenido de ADN fue 2,29 pg para el genotipo con número cromosómico 2n = 24 y 4,03 pg para el genotipo 2n = 48. Se encontró un efecto significativo (α = 0,01) del número de cromosomas sobre el contenido de ADN nuclear para las dos especies. Uchuva-Physalis peruviana L. 2019-08-09T19:30:08Z 2019-08-09T19:30:08Z 2014 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 http://revistacta.agrosavia.co/index.php/revista/article/view/396 10.21930/rcta.vol15_num1_art:396 http://hdl.handle.net/20.500.12324/35186 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA spa http://revistacta.agrosavia.co/index.php/revista/article/view/396/313 Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ info:eu-repo/semantics/openAccess application/pdf application/pdf Corporación colombiana de investigación agropecuaria - AGROSAVIA Revista Ciencia y Tecnología Agropecuaria; Vol 15, Núm 1 (2014); Ciencia y tecnología agropecuaria; p.51-61. |
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Corporación Colombiana de Investigación Agropecuaria |
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Repositorio AGROSAVIA |
| language |
Español |
| topic |
Frutales |
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Frutales Liberato Guío, Sara Alejandra Sánchez Betancourt, Érika Patricia Arguelles Cárdenas, Jorge González Almario, Carolina Núñez Zarante, Víctor Manuel Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum |
| description |
Vascular wilt caused by the fungus Fusarium oxysporum is considered the main constraint of cape gooseberry, Physalis peruviana, production in Colombia. P. peruviana and P. floridana genotypes with differential resistance responses against F. oxysporum have been identified previously. In the present study, the genotypes were evaluated in order to complement the knowledge of cytogenetics diversity in Physalis and to design hybridization strategies to support breeding of cape gooseberry crop. The chromosome number in mitotic dividing cells from root-tips of tissue culture plantlets was determined, from which the average mitotic hour was estimated at 12:00 hours for P. peruviana and 10:00 for P. floridana. Chromosomic complements of 2n = 4x = 48 and 2n = 2x = 24 were found for each one of the two species. Additionally, flow cytometry analyses detected variation within P. peruviana with a nuclear DNA content of 2.33 pg for the 2n = 24 genotype and variations ranged from 5.77 to 8.12 pg for 2n = 48 genotypes. In P. floridana DNA content was 2.29 pg in the 2n = 24 genotype and 4.03 pg in the 2n = 48 genotype. There was a significant effect (α = 0.01) of the number of chromosomes on nuclear DNA content for the two species |
| format |
article |
| author |
Liberato Guío, Sara Alejandra Sánchez Betancourt, Érika Patricia Arguelles Cárdenas, Jorge González Almario, Carolina Núñez Zarante, Víctor Manuel |
| author_facet |
Liberato Guío, Sara Alejandra Sánchez Betancourt, Érika Patricia Arguelles Cárdenas, Jorge González Almario, Carolina Núñez Zarante, Víctor Manuel |
| author_sort |
Liberato Guío, Sara Alejandra |
| title |
Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum |
| title_short |
Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum |
| title_full |
Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum |
| title_fullStr |
Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum |
| title_full_unstemmed |
Cytogenetics of Physalis peruviana L. and Physalis floridana Rydb. genotypes with differential response to Fusarium oxysporum |
| title_sort |
cytogenetics of physalis peruviana l. and physalis floridana rydb. genotypes with differential response to fusarium oxysporum |
| publisher |
Corporación colombiana de investigación agropecuaria - AGROSAVIA |
| publishDate |
2019 |
| url |
http://revistacta.agrosavia.co/index.php/revista/article/view/396 http://hdl.handle.net/20.500.12324/35186 |
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