First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil
The state of Santa Catarina (SC), southern Brazil, is estimated to have nearly 100 hectares dedicated to olive (Olea europaea L.) cultivation, with promising growth prospects. Herein, we report the first detection of Xylella fastidiosa subsp. pauca (Xfp) in olive trees in SC. Xfp was initially...
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| Format: | publishedVersion |
| Language: | Inglés |
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Springer
2025
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| Online Access: | https://hdl.handle.net/20.500.11939/9064 https://link.springer.com/article/10.1007/s42161-025-01894-2 |
| _version_ | 1855032905170944000 |
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| author | Canale, Maria Cristina Cardoza, Yuliet Chaves Ortiz, Pamella Mituti, Tatiana Fernandes, Juliane Roveri Sabiäo, Rafael Barbé, Silvia Brugnara, Eduardo Cesar |
| author_browse | Barbé, Silvia Brugnara, Eduardo Cesar Canale, Maria Cristina Cardoza, Yuliet Chaves Ortiz, Pamella Fernandes, Juliane Mituti, Tatiana Roveri Sabiäo, Rafael |
| author_facet | Canale, Maria Cristina Cardoza, Yuliet Chaves Ortiz, Pamella Mituti, Tatiana Fernandes, Juliane Roveri Sabiäo, Rafael Barbé, Silvia Brugnara, Eduardo Cesar |
| author_sort | Canale, Maria Cristina |
| collection | ReDivia |
| description | The state of Santa Catarina (SC), southern Brazil, is estimated
to have nearly 100 hectares dedicated to olive (Olea
europaea L.) cultivation, with promising growth prospects.
Herein, we report the first detection of Xylella fastidiosa
subsp. pauca (Xfp) in olive trees in SC. Xfp was initially
identified to cause citrus variegated chlorosis and coffee leaf
scorch in Brazil. Since 2013, it has been associated with
the Olive Quick Decline Syndrome in Italy, and soon after,
in Brazil (Safady et al. 2019). Three trees cv. ‘Koroneiki’
and one cv. ‘Arbequina’, aged 6 years old, exhibited leaf browning and scorch symptoms, along with premature twig
and branch dieback (Supplemental Fig. 1), in an experimental
orchard in Chapecó. Symptomatic twig samples from
those plants and one sample from an asymptomatic ‘Koroneiki’
tree were collected in springer 2023 and summer
2024 and were subjected to DNA extraction using Wizard
DNA purification kit (Promega) and PCR amplification of
seven housekeeping genes (leuA, petC, malF, cysG, holC,
nuoL, gltT) (Yuan et al. 2010). Amplicons were purified and
sequenced on both strands. The sequences were edited using
the BioEdit 7.0.5.3 software, and consensus sequences were
analyzed using the MEGA software. The results of sequencing
were compared with available sequences on PubMLST
(Jolley et al. 2018). Approximate amplicon size, adjusted
without primers, and the allele number identified were:
670 bp for leuA (allele 7), 551 bp for petC (allele 6), 685 bp
for malF (allele 7), 608 bp for cysG (allele 9), 399 bp for
holC (allele 23), 578 bp for nuoL (allele 17) and 635 bp for
gltT (allele 8), which correspond to sequence type (ST) 69
of Xf subspecies pauca [(isolate OliveECB01, PubMLST
id:31) (GenBank accession numbers PQ550632-38, respectively
to each gene)]. Xfp ST 69 isolates were previously
found in Citrus sinensis in Argentina (Coletta-Filho et al.
2017), next to the border with Santa Catarina. This is the
first occurrence of this sequence type in Brazil |
| format | publishedVersion |
| id | ReDivia9064 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia90642025-05-27T08:47:37Z First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil Canale, Maria Cristina Cardoza, Yuliet Chaves Ortiz, Pamella Mituti, Tatiana Fernandes, Juliane Roveri Sabiäo, Rafael Barbé, Silvia Brugnara, Eduardo Cesar Multilocus sequence typing (MLST) Xylella variability Xylella epidemiology H20 Plant diseases The state of Santa Catarina (SC), southern Brazil, is estimated to have nearly 100 hectares dedicated to olive (Olea europaea L.) cultivation, with promising growth prospects. Herein, we report the first detection of Xylella fastidiosa subsp. pauca (Xfp) in olive trees in SC. Xfp was initially identified to cause citrus variegated chlorosis and coffee leaf scorch in Brazil. Since 2013, it has been associated with the Olive Quick Decline Syndrome in Italy, and soon after, in Brazil (Safady et al. 2019). Three trees cv. ‘Koroneiki’ and one cv. ‘Arbequina’, aged 6 years old, exhibited leaf browning and scorch symptoms, along with premature twig and branch dieback (Supplemental Fig. 1), in an experimental orchard in Chapecó. Symptomatic twig samples from those plants and one sample from an asymptomatic ‘Koroneiki’ tree were collected in springer 2023 and summer 2024 and were subjected to DNA extraction using Wizard DNA purification kit (Promega) and PCR amplification of seven housekeeping genes (leuA, petC, malF, cysG, holC, nuoL, gltT) (Yuan et al. 2010). Amplicons were purified and sequenced on both strands. The sequences were edited using the BioEdit 7.0.5.3 software, and consensus sequences were analyzed using the MEGA software. The results of sequencing were compared with available sequences on PubMLST (Jolley et al. 2018). Approximate amplicon size, adjusted without primers, and the allele number identified were: 670 bp for leuA (allele 7), 551 bp for petC (allele 6), 685 bp for malF (allele 7), 608 bp for cysG (allele 9), 399 bp for holC (allele 23), 578 bp for nuoL (allele 17) and 635 bp for gltT (allele 8), which correspond to sequence type (ST) 69 of Xf subspecies pauca [(isolate OliveECB01, PubMLST id:31) (GenBank accession numbers PQ550632-38, respectively to each gene)]. Xfp ST 69 isolates were previously found in Citrus sinensis in Argentina (Coletta-Filho et al. 2017), next to the border with Santa Catarina. This is the first occurrence of this sequence type in Brazil 2025-05-27T08:47:36Z 2025-05-27T08:47:36Z 2025 publishedVersion Canale, M. C., Cardoza, Y., Ortiz, P. C., Mituti, T., Fernandes, J., Sabião, R. R., ... & Brugnara, E. C. (2025). First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil. Journal of Plant Pathology, 107(2), 1311-1312. 1125-4653 https://hdl.handle.net/20.500.11939/9064 10.1007/s42161-025-01894-2 https://link.springer.com/article/10.1007/s42161-025-01894-2 en Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Springer |
| spellingShingle | Multilocus sequence typing (MLST) Xylella variability Xylella epidemiology H20 Plant diseases Canale, Maria Cristina Cardoza, Yuliet Chaves Ortiz, Pamella Mituti, Tatiana Fernandes, Juliane Roveri Sabiäo, Rafael Barbé, Silvia Brugnara, Eduardo Cesar First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil |
| title | First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil |
| title_full | First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil |
| title_fullStr | First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil |
| title_full_unstemmed | First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil |
| title_short | First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil |
| title_sort | first report of xylella fastidiosa subsp pauca in olea europaea in santa catarina southern brazil |
| topic | Multilocus sequence typing (MLST) Xylella variability Xylella epidemiology H20 Plant diseases |
| url | https://hdl.handle.net/20.500.11939/9064 https://link.springer.com/article/10.1007/s42161-025-01894-2 |
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