First report of Xylella fastidiosa subsp. pauca in Olea europaea in Santa Catarina, Southern Brazil
The state of Santa Catarina (SC), southern Brazil, is estimated to have nearly 100 hectares dedicated to olive (Olea europaea L.) cultivation, with promising growth prospects. Herein, we report the first detection of Xylella fastidiosa subsp. pauca (Xfp) in olive trees in SC. Xfp was initially...
| Autores principales: | , , , , , , , |
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| Formato: | publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9064 https://link.springer.com/article/10.1007/s42161-025-01894-2 |
| Sumario: | The state of Santa Catarina (SC), southern Brazil, is estimated
to have nearly 100 hectares dedicated to olive (Olea
europaea L.) cultivation, with promising growth prospects.
Herein, we report the first detection of Xylella fastidiosa
subsp. pauca (Xfp) in olive trees in SC. Xfp was initially
identified to cause citrus variegated chlorosis and coffee leaf
scorch in Brazil. Since 2013, it has been associated with
the Olive Quick Decline Syndrome in Italy, and soon after,
in Brazil (Safady et al. 2019). Three trees cv. ‘Koroneiki’
and one cv. ‘Arbequina’, aged 6 years old, exhibited leaf browning and scorch symptoms, along with premature twig
and branch dieback (Supplemental Fig. 1), in an experimental
orchard in Chapecó. Symptomatic twig samples from
those plants and one sample from an asymptomatic ‘Koroneiki’
tree were collected in springer 2023 and summer
2024 and were subjected to DNA extraction using Wizard
DNA purification kit (Promega) and PCR amplification of
seven housekeeping genes (leuA, petC, malF, cysG, holC,
nuoL, gltT) (Yuan et al. 2010). Amplicons were purified and
sequenced on both strands. The sequences were edited using
the BioEdit 7.0.5.3 software, and consensus sequences were
analyzed using the MEGA software. The results of sequencing
were compared with available sequences on PubMLST
(Jolley et al. 2018). Approximate amplicon size, adjusted
without primers, and the allele number identified were:
670 bp for leuA (allele 7), 551 bp for petC (allele 6), 685 bp
for malF (allele 7), 608 bp for cysG (allele 9), 399 bp for
holC (allele 23), 578 bp for nuoL (allele 17) and 635 bp for
gltT (allele 8), which correspond to sequence type (ST) 69
of Xf subspecies pauca [(isolate OliveECB01, PubMLST
id:31) (GenBank accession numbers PQ550632-38, respectively
to each gene)]. Xfp ST 69 isolates were previously
found in Citrus sinensis in Argentina (Coletta-Filho et al.
2017), next to the border with Santa Catarina. This is the
first occurrence of this sequence type in Brazil |
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