Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants
Parietaria mottle virus (genus Ilarvirus, family Bromoviridae) is an emerging virus infecting tomato and pepper crops in the Mediterranean basin. PMoV has a segmented single-stranded positivesense RNA genome encoding two replicase subunits (p1 and p2), the 2b, the movement (MP) and coat (CP) p...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9039 https://link.springer.com/article/10.1007/s10658-025-03002-7 |
| Sumario: | Parietaria mottle virus (genus Ilarvirus,
family Bromoviridae) is an emerging virus infecting
tomato and pepper crops in the Mediterranean basin.
PMoV has a segmented single-stranded positivesense
RNA genome encoding two replicase subunits
(p1 and p2), the 2b, the movement (MP) and coat
(CP) proteins. Mechanisms underlying the disease
development are poorly understood in most virusplant
pathosystems. For this purpose, we have investigated
the role played by the PMoV CP, MP, and 2b
as pathogen determinants in the experimental host
Nicotiana benthamiana. Transient expression of the
PMoV proteins by using the PVX cDNA viral vector
pGR107 showed that all of them enhanced the PVXinduced
symptoms in N. benthamiana agroinfiltrated
plants, but with different degrees. CP was associated
with strong symptoms of systemic necrosis typical of
hypersensitive host response (HR), mosaic leaf deformation,
and plant stunting. 2b and MP were associated
with mild mosaic and leaf deformation. However,
movement complementation assays of a viral vector
based on turnip crinkle virus sequence expressing
GFP (pTCV-GFP) revealed that none of these PMoV
proteins could suppress the post-transcriptional gene
silencing (PTGS) host defense mechanism. Additional
assays of systemic RNA silencing in transgenic
N. benthamiana 16c plants expressing the green
fluorescent protein (GFP) confirmed that PMoV 2b,
unlike other ilarviruses, was not a PTGS suppressor.
Results obtained here are discussed and compared
with those of other virus-plant pathosystems |
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