Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants
Parietaria mottle virus (genus Ilarvirus, family Bromoviridae) is an emerging virus infecting tomato and pepper crops in the Mediterranean basin. PMoV has a segmented single-stranded positivesense RNA genome encoding two replicase subunits (p1 and p2), the 2b, the movement (MP) and coat (CP) p...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9039 https://link.springer.com/article/10.1007/s10658-025-03002-7 |
| _version_ | 1855032900344348672 |
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| author | Martínez, C. López, C. Pallás, Vicente Aparicio, Frederic Galipienso, Luis |
| author_browse | Aparicio, Frederic Galipienso, Luis López, C. Martínez, C. Pallás, Vicente |
| author_facet | Martínez, C. López, C. Pallás, Vicente Aparicio, Frederic Galipienso, Luis |
| author_sort | Martínez, C. |
| collection | ReDivia |
| description | Parietaria mottle virus (genus Ilarvirus,
family Bromoviridae) is an emerging virus infecting
tomato and pepper crops in the Mediterranean basin.
PMoV has a segmented single-stranded positivesense
RNA genome encoding two replicase subunits
(p1 and p2), the 2b, the movement (MP) and coat
(CP) proteins. Mechanisms underlying the disease
development are poorly understood in most virusplant
pathosystems. For this purpose, we have investigated
the role played by the PMoV CP, MP, and 2b
as pathogen determinants in the experimental host
Nicotiana benthamiana. Transient expression of the
PMoV proteins by using the PVX cDNA viral vector
pGR107 showed that all of them enhanced the PVXinduced
symptoms in N. benthamiana agroinfiltrated
plants, but with different degrees. CP was associated
with strong symptoms of systemic necrosis typical of
hypersensitive host response (HR), mosaic leaf deformation,
and plant stunting. 2b and MP were associated
with mild mosaic and leaf deformation. However,
movement complementation assays of a viral vector
based on turnip crinkle virus sequence expressing
GFP (pTCV-GFP) revealed that none of these PMoV
proteins could suppress the post-transcriptional gene
silencing (PTGS) host defense mechanism. Additional
assays of systemic RNA silencing in transgenic
N. benthamiana 16c plants expressing the green
fluorescent protein (GFP) confirmed that PMoV 2b,
unlike other ilarviruses, was not a PTGS suppressor.
Results obtained here are discussed and compared
with those of other virus-plant pathosystems |
| format | article |
| id | ReDivia9039 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia90392025-04-25T14:49:52Z Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants Martínez, C. López, C. Pallás, Vicente Aparicio, Frederic Galipienso, Luis PMoV Nicotiana benthamiana Pathogen determinants PTGS PGR107 GFP H20 Plant diseases Parietaria mottle virus (genus Ilarvirus, family Bromoviridae) is an emerging virus infecting tomato and pepper crops in the Mediterranean basin. PMoV has a segmented single-stranded positivesense RNA genome encoding two replicase subunits (p1 and p2), the 2b, the movement (MP) and coat (CP) proteins. Mechanisms underlying the disease development are poorly understood in most virusplant pathosystems. For this purpose, we have investigated the role played by the PMoV CP, MP, and 2b as pathogen determinants in the experimental host Nicotiana benthamiana. Transient expression of the PMoV proteins by using the PVX cDNA viral vector pGR107 showed that all of them enhanced the PVXinduced symptoms in N. benthamiana agroinfiltrated plants, but with different degrees. CP was associated with strong symptoms of systemic necrosis typical of hypersensitive host response (HR), mosaic leaf deformation, and plant stunting. 2b and MP were associated with mild mosaic and leaf deformation. However, movement complementation assays of a viral vector based on turnip crinkle virus sequence expressing GFP (pTCV-GFP) revealed that none of these PMoV proteins could suppress the post-transcriptional gene silencing (PTGS) host defense mechanism. Additional assays of systemic RNA silencing in transgenic N. benthamiana 16c plants expressing the green fluorescent protein (GFP) confirmed that PMoV 2b, unlike other ilarviruses, was not a PTGS suppressor. Results obtained here are discussed and compared with those of other virus-plant pathosystems 2025-03-11T08:52:09Z 2025-03-11T08:52:09Z 2025 article publishedVersion Martínez, C., López, C., Pallás, V., Aparicio, F., & Galipienso, L. (2025). Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants. European Journal of Plant Pathology. 0929-1873 https://hdl.handle.net/20.500.11939/9039 10.1007/s10658-025-03002-7 https://link.springer.com/article/10.1007/s10658-025-03002-7 en This work was supported in part by the project RTA2006-00024-C02-01 funded by the Spanish agency Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and by grant TED2021-131949b-100 funded by MCIN/AEI/https:// doi. org/ 10. 13039/ 50110 00110 33 and the “NextGenerationEU”/PRTR from the European Union. Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Springer electronico |
| spellingShingle | PMoV Nicotiana benthamiana Pathogen determinants PTGS PGR107 GFP H20 Plant diseases Martínez, C. López, C. Pallás, Vicente Aparicio, Frederic Galipienso, Luis Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants |
| title | Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants |
| title_full | Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants |
| title_fullStr | Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants |
| title_full_unstemmed | Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants |
| title_short | Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants |
| title_sort | role of the coat cp movement mp and 2b proteins of parietaria motte virus pmov as pathogen determinants in nicotiana benthamiana plants |
| topic | PMoV Nicotiana benthamiana Pathogen determinants PTGS PGR107 GFP H20 Plant diseases |
| url | https://hdl.handle.net/20.500.11939/9039 https://link.springer.com/article/10.1007/s10658-025-03002-7 |
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