Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection
Tomato is one of the most important crops worldwide, with a production of ≈190 million tons, but it is constantly threatened by several viral diseases. Tomato brown rugose fruit virus (ToBRFV), identified in 2014 on tomato plants and subsequently reported in many countries, represents one of the...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9019 https://www.mdpi.com/2073-4395/14/3/412 |
| _version_ | 1855492608311164928 |
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| author | Caruso, A. G. Panno, Stefano Ragona, Arianna Peiró, Rosa Vetrano, Filippo Moncada, Alessandra Miceli, Alessandro La Marra, Consuelo Maria Galipienso, Luis Rubio, Luis Davino, Salvatore |
| author_browse | Caruso, A. G. Davino, Salvatore Galipienso, Luis La Marra, Consuelo Maria Miceli, Alessandro Moncada, Alessandra Panno, Stefano Peiró, Rosa Ragona, Arianna Rubio, Luis Vetrano, Filippo |
| author_facet | Caruso, A. G. Panno, Stefano Ragona, Arianna Peiró, Rosa Vetrano, Filippo Moncada, Alessandra Miceli, Alessandro La Marra, Consuelo Maria Galipienso, Luis Rubio, Luis Davino, Salvatore |
| author_sort | Caruso, A. G. |
| collection | ReDivia |
| description | Tomato is one of the most important crops worldwide, with a production of ≈190 million tons,
but it is constantly threatened by several viral diseases. Tomato brown rugose fruit virus (ToBRFV),
identified in 2014 on tomato plants and subsequently reported in many countries, represents one of
the major threats to tomato crops, due to production losses, different transmission modes and its rapid
spread. This work aimed to evaluate 37 local Sicilian tomato ecotypes against ToBRFV infection. After a
preliminary screening by molecular analyses for tomato mosaic virus (ToMV) and pepino mosaic virus
(PepMV), and ToBRFV detection, tomato plants were grown in a greenhouse for their morphological
characterization and for evaluating resistance and tolerance to ToBRFV. Resistance and tolerance levels
were estimated by mechanical inoculation with ToB SIC01/19 ToBRFV isolate in ten plants per ecotype
and evaluating virus accumulation by RT-qPCR and visual observation of symptoms. All ecotypes were
infected with ToBRFV, showing several symptoms with different disease severity. No tomato ecotype
showed a high level of resistance, but two ecotypes, Pop27 and Pop35, showed very moderate symptoms
and therefore a high tolerance. These Sicilian tomato ecotypes could be used in genetic breeding programs
as parental ones to obtain cultivars tolerant to ToBRFV. |
| format | Artículo |
| id | ReDivia9019 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia90192025-04-25T14:49:46Z Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection Caruso, A. G. Panno, Stefano Ragona, Arianna Peiró, Rosa Vetrano, Filippo Moncada, Alessandra Miceli, Alessandro La Marra, Consuelo Maria Galipienso, Luis Rubio, Luis Davino, Salvatore ToBRFV Virus tolerance H20 Plant diseases Tomatoes Tobamoviruses Ecotypes Tomato is one of the most important crops worldwide, with a production of ≈190 million tons, but it is constantly threatened by several viral diseases. Tomato brown rugose fruit virus (ToBRFV), identified in 2014 on tomato plants and subsequently reported in many countries, represents one of the major threats to tomato crops, due to production losses, different transmission modes and its rapid spread. This work aimed to evaluate 37 local Sicilian tomato ecotypes against ToBRFV infection. After a preliminary screening by molecular analyses for tomato mosaic virus (ToMV) and pepino mosaic virus (PepMV), and ToBRFV detection, tomato plants were grown in a greenhouse for their morphological characterization and for evaluating resistance and tolerance to ToBRFV. Resistance and tolerance levels were estimated by mechanical inoculation with ToB SIC01/19 ToBRFV isolate in ten plants per ecotype and evaluating virus accumulation by RT-qPCR and visual observation of symptoms. All ecotypes were infected with ToBRFV, showing several symptoms with different disease severity. No tomato ecotype showed a high level of resistance, but two ecotypes, Pop27 and Pop35, showed very moderate symptoms and therefore a high tolerance. These Sicilian tomato ecotypes could be used in genetic breeding programs as parental ones to obtain cultivars tolerant to ToBRFV. 2025-01-20T13:17:59Z 2025-01-20T13:17:59Z 2024 article publishedVersion Caruso, A. G., Panno, S., Ragona, A., Peiró, R., Vetrano, F., Moncada, A., ... & Davino, S. (2024). Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection. Agronomy, 14(3), 412. 2073-4395 https://hdl.handle.net/20.500.11939/9019 10.3390/agronomy14030412 https://www.mdpi.com/2073-4395/14/3/412 en info:eu-repo/grantAgreement/AEI/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia/PID2021-125787OR-C31/ES/DETECCION Y CONTROL DE VIRUS EMERGENTES DE CUCURBITACEAS Y SOLANACEAS EN LA CUENCA MEDITERRANEA This research was funded by the MUR (Ministero dell’Università e della Ricerca)-PNRRM4C2- ECS_00000022 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | ToBRFV Virus tolerance H20 Plant diseases Tomatoes Tobamoviruses Ecotypes Caruso, A. G. Panno, Stefano Ragona, Arianna Peiró, Rosa Vetrano, Filippo Moncada, Alessandra Miceli, Alessandro La Marra, Consuelo Maria Galipienso, Luis Rubio, Luis Davino, Salvatore Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection |
| title | Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection |
| title_full | Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection |
| title_fullStr | Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection |
| title_full_unstemmed | Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection |
| title_short | Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection |
| title_sort | screening local sicilian tomato ecotypes to evaluate the response of tomato brown rugose fruit virus infection |
| topic | ToBRFV Virus tolerance H20 Plant diseases Tomatoes Tobamoviruses Ecotypes |
| url | https://hdl.handle.net/20.500.11939/9019 https://www.mdpi.com/2073-4395/14/3/412 |
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