Screening Local Sicilian Tomato Ecotypes to Evaluate the Response of Tomato Brown Rugose Fruit Virus Infection
Tomato is one of the most important crops worldwide, with a production of ≈190 million tons, but it is constantly threatened by several viral diseases. Tomato brown rugose fruit virus (ToBRFV), identified in 2014 on tomato plants and subsequently reported in many countries, represents one of the...
| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9019 https://www.mdpi.com/2073-4395/14/3/412 |
| Sumario: | Tomato is one of the most important crops worldwide, with a production of ≈190 million tons,
but it is constantly threatened by several viral diseases. Tomato brown rugose fruit virus (ToBRFV),
identified in 2014 on tomato plants and subsequently reported in many countries, represents one of
the major threats to tomato crops, due to production losses, different transmission modes and its rapid
spread. This work aimed to evaluate 37 local Sicilian tomato ecotypes against ToBRFV infection. After a
preliminary screening by molecular analyses for tomato mosaic virus (ToMV) and pepino mosaic virus
(PepMV), and ToBRFV detection, tomato plants were grown in a greenhouse for their morphological
characterization and for evaluating resistance and tolerance to ToBRFV. Resistance and tolerance levels
were estimated by mechanical inoculation with ToB SIC01/19 ToBRFV isolate in ten plants per ecotype
and evaluating virus accumulation by RT-qPCR and visual observation of symptoms. All ecotypes were
infected with ToBRFV, showing several symptoms with different disease severity. No tomato ecotype
showed a high level of resistance, but two ecotypes, Pop27 and Pop35, showed very moderate symptoms
and therefore a high tolerance. These Sicilian tomato ecotypes could be used in genetic breeding programs
as parental ones to obtain cultivars tolerant to ToBRFV. |
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