Intraspecific diversity of Erwinia amylovora strains from northern Algeria
Background Fire blight, caused by Erwinia amylovora, is the most destructive bacterial disease affecting plants in the Rosaceae family, leading to significant economic losses. In Algeria, this disease has been reported since 2010. This study aimed to investigate the origin of fire blight in Algeri...
| Main Authors: | , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.11939/8998 https://link.springer.com/article/10.1186/s12866-024-03555-3 |
| _version_ | 1855492604153561088 |
|---|---|
| author | Talhi, Lina Barbé, Silvia Navarro-Herrero, Inmaculada Sebaihia, Mohammed Marco-Noales, Ester |
| author_browse | Barbé, Silvia Marco-Noales, Ester Navarro-Herrero, Inmaculada Sebaihia, Mohammed Talhi, Lina |
| author_facet | Talhi, Lina Barbé, Silvia Navarro-Herrero, Inmaculada Sebaihia, Mohammed Marco-Noales, Ester |
| author_sort | Talhi, Lina |
| collection | ReDivia |
| description | Background Fire blight, caused by Erwinia amylovora, is the most destructive bacterial disease affecting plants in
the Rosaceae family, leading to significant economic losses. In Algeria, this disease has been reported since 2010. This
study aimed to investigate the origin of fire blight in Algeria, in order to increase knowledge of the epidemiology of
this serious disease and contribute to its management. A comprehensive characterization of 18 E. amylovora isolates
recovered from northern Algeria between 2016 and 2021 to evaluate their phenotypical and genotypical diversity
was conducted.
Results Phenotypic differences, particularly in growth kinetics, virulence, and fatty acid profiles, allowed
differentiation of strains into five groups, possibly indicating distinct introduction events. Genetic characterization
revealed that only one strain lacked the ubiquitous plasmid pEA29, which is correlated with reduced virulence, while
none harbored the pEI70 plasmid. Phylogenetic analysis using concatenated sequences of the recA, groEL, rpoS, ams,
and hrpN genes grouped Algerian strains with those from a broadly prevalent clade. CRISPR genotyping identified a
novel CR1 pattern and three genotypes, two of them previously unreported.
Conclusions This study represents the first phenotypic, genetic, and phylogenetic investigation of E. amylovora
strains in the region, and provides valuable information on the possible pathways of the introduction of this fire blight
pathogen in northern Africa. The findings suggest one or more introduction events from a common ancestor, likely
originating in northern Italy, followed by dispersal in various regions of Algeria. |
| format | Artículo |
| id | ReDivia8998 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia89982025-04-25T14:49:42Z Intraspecific diversity of Erwinia amylovora strains from northern Algeria Talhi, Lina Barbé, Silvia Navarro-Herrero, Inmaculada Sebaihia, Mohammed Marco-Noales, Ester Diversity Growth kinetics Virulence Fatty acid profile CRISPR MLST H20 Plant diseases Erwinia amylovora Fire blight Plasmids Background Fire blight, caused by Erwinia amylovora, is the most destructive bacterial disease affecting plants in the Rosaceae family, leading to significant economic losses. In Algeria, this disease has been reported since 2010. This study aimed to investigate the origin of fire blight in Algeria, in order to increase knowledge of the epidemiology of this serious disease and contribute to its management. A comprehensive characterization of 18 E. amylovora isolates recovered from northern Algeria between 2016 and 2021 to evaluate their phenotypical and genotypical diversity was conducted. Results Phenotypic differences, particularly in growth kinetics, virulence, and fatty acid profiles, allowed differentiation of strains into five groups, possibly indicating distinct introduction events. Genetic characterization revealed that only one strain lacked the ubiquitous plasmid pEA29, which is correlated with reduced virulence, while none harbored the pEI70 plasmid. Phylogenetic analysis using concatenated sequences of the recA, groEL, rpoS, ams, and hrpN genes grouped Algerian strains with those from a broadly prevalent clade. CRISPR genotyping identified a novel CR1 pattern and three genotypes, two of them previously unreported. Conclusions This study represents the first phenotypic, genetic, and phylogenetic investigation of E. amylovora strains in the region, and provides valuable information on the possible pathways of the introduction of this fire blight pathogen in northern Africa. The findings suggest one or more introduction events from a common ancestor, likely originating in northern Italy, followed by dispersal in various regions of Algeria. 2024-10-25T08:38:04Z 2024-10-25T08:38:04Z 2024 article publishedVersion Talhi, L., Barbé, S., Navarro-Herrero, I., Sebaihia, M., & Marco-Noales, E. (2024). Intraspecific diversity of Erwinia amylovora strains from northern Algeria. BMC microbiology, 24(1), 389. 1471-2180 https://hdl.handle.net/20.500.11939/8998 10.1186/s12866-024-03555-3 https://link.springer.com/article/10.1186/s12866-024-03555-3 en This study was funded by the Algerian Ministry of Higher Education and Scientific Research (DGRSDT/MESRS) and by the project IVIA-GVA 52202D from Instituto Valenciano de Investigaciones Agrarias (project susceptible to being co-financed by the European Union through the ERDF Program 2021–2027 Comunitat Valenciana). info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52202/ES/Control biológico de plagas y enfermedades como pieza clave en el impulso de la Sostenibilidad de los cultivos agrarios de la Comunidad Valenciana/SOSTENIBLE Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Springer electronico |
| spellingShingle | Diversity Growth kinetics Virulence Fatty acid profile CRISPR MLST H20 Plant diseases Erwinia amylovora Fire blight Plasmids Talhi, Lina Barbé, Silvia Navarro-Herrero, Inmaculada Sebaihia, Mohammed Marco-Noales, Ester Intraspecific diversity of Erwinia amylovora strains from northern Algeria |
| title | Intraspecific diversity of Erwinia amylovora strains from northern Algeria |
| title_full | Intraspecific diversity of Erwinia amylovora strains from northern Algeria |
| title_fullStr | Intraspecific diversity of Erwinia amylovora strains from northern Algeria |
| title_full_unstemmed | Intraspecific diversity of Erwinia amylovora strains from northern Algeria |
| title_short | Intraspecific diversity of Erwinia amylovora strains from northern Algeria |
| title_sort | intraspecific diversity of erwinia amylovora strains from northern algeria |
| topic | Diversity Growth kinetics Virulence Fatty acid profile CRISPR MLST H20 Plant diseases Erwinia amylovora Fire blight Plasmids |
| url | https://hdl.handle.net/20.500.11939/8998 https://link.springer.com/article/10.1186/s12866-024-03555-3 |
| work_keys_str_mv | AT talhilina intraspecificdiversityoferwiniaamylovorastrainsfromnorthernalgeria AT barbesilvia intraspecificdiversityoferwiniaamylovorastrainsfromnorthernalgeria AT navarroherreroinmaculada intraspecificdiversityoferwiniaamylovorastrainsfromnorthernalgeria AT sebaihiamohammed intraspecificdiversityoferwiniaamylovorastrainsfromnorthernalgeria AT marconoalesester intraspecificdiversityoferwiniaamylovorastrainsfromnorthernalgeria |