Intraspecific diversity of Erwinia amylovora strains from northern Algeria
Background Fire blight, caused by Erwinia amylovora, is the most destructive bacterial disease affecting plants in the Rosaceae family, leading to significant economic losses. In Algeria, this disease has been reported since 2010. This study aimed to investigate the origin of fire blight in Algeri...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8998 https://link.springer.com/article/10.1186/s12866-024-03555-3 |
| Sumario: | Background Fire blight, caused by Erwinia amylovora, is the most destructive bacterial disease affecting plants in
the Rosaceae family, leading to significant economic losses. In Algeria, this disease has been reported since 2010. This
study aimed to investigate the origin of fire blight in Algeria, in order to increase knowledge of the epidemiology of
this serious disease and contribute to its management. A comprehensive characterization of 18 E. amylovora isolates
recovered from northern Algeria between 2016 and 2021 to evaluate their phenotypical and genotypical diversity
was conducted.
Results Phenotypic differences, particularly in growth kinetics, virulence, and fatty acid profiles, allowed
differentiation of strains into five groups, possibly indicating distinct introduction events. Genetic characterization
revealed that only one strain lacked the ubiquitous plasmid pEA29, which is correlated with reduced virulence, while
none harbored the pEI70 plasmid. Phylogenetic analysis using concatenated sequences of the recA, groEL, rpoS, ams,
and hrpN genes grouped Algerian strains with those from a broadly prevalent clade. CRISPR genotyping identified a
novel CR1 pattern and three genotypes, two of them previously unreported.
Conclusions This study represents the first phenotypic, genetic, and phylogenetic investigation of E. amylovora
strains in the region, and provides valuable information on the possible pathways of the introduction of this fire blight
pathogen in northern Africa. The findings suggest one or more introduction events from a common ancestor, likely
originating in northern Italy, followed by dispersal in various regions of Algeria. |
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