Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions
The microbial population in the pig’s gastrointestinal tract can be influenced by incorporating fibrous by-products into the diets. This study investigated the impact of including two types of dried olive cake (OC) in pigs’ diets on fecal bacterial composition. The correlation between fecal microbio...
| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8952 https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1359670/full |
| _version_ | 1855032884591591424 |
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| author | Belloumi, Dhekra García-Rebollar, Paloma Calvet, Salvador Francino, P. Reyes-Prieto, Mariana González-Garrido, Jorge Piquer, Laia Jiménez-Belenguer, Ana Isabel Bermejo, Almudena Cano, Carmen Cerisuelo, Alba |
| author_browse | Belloumi, Dhekra Bermejo, Almudena Calvet, Salvador Cano, Carmen Cerisuelo, Alba Francino, P. García-Rebollar, Paloma González-Garrido, Jorge Jiménez-Belenguer, Ana Isabel Piquer, Laia Reyes-Prieto, Mariana |
| author_facet | Belloumi, Dhekra García-Rebollar, Paloma Calvet, Salvador Francino, P. Reyes-Prieto, Mariana González-Garrido, Jorge Piquer, Laia Jiménez-Belenguer, Ana Isabel Bermejo, Almudena Cano, Carmen Cerisuelo, Alba |
| author_sort | Belloumi, Dhekra |
| collection | ReDivia |
| description | The microbial population in the pig’s gastrointestinal tract can be influenced by incorporating fibrous by-products into the diets. This study investigated the impact of including two types of dried olive cake (OC) in pigs’ diets on fecal bacterial composition. The correlation between fecal microbiota and growth performance, nutrient digestibility, gut fermentation pattern and slurry gas emissions was also evaluated. Thirty male Pietrain x (Landrace x Large white) pigs (47.9 ± 4.21 kg) were assigned to three groups: a control group (C), a group fed a diet with 20% partially defatted OC (20PDOC), and a group fed a diet with 20% cyclone OC (20COC) for 21 days. Fecal samples collected before and after providing the experimental diets were analyzed for the V3-V4 region of the 16S rRNA gene. Pigs were weighed, and feed intake was recorded throughout the study. Potential ammonia and methane emissions from slurry were measured. No significant differences in alpha diversity indexes were found. The taxonomic analysis revealed that Firmicutes and Bacteroidota phyla were dominant at the phylum level across all groups. Differential abundance analysis using ALDEx showed significant differences among groups for various bacteria at the phylum, genus, and species levels at the end of the experiment. Pigs from 20PDOC and 20COC groups exhibited increased abundances of health-promoting bacteria, such as Plactomycetota at the phylum level and Allisonella and an unidentified genus from the Eggerthellaceae family at the genus level. These changes influenced short-chain fatty acids’ (SCFA) concentration in slurries, leading to greater acetic, butyric, caproic and heptanoic acids in OC-fed groups, especially 20COC pigs. A volatility analysis revealed significant positive correlations (p < 0.05) between Uncultured_Bacteroidales and Unculured_Selenomonadaceae and energy digestibility. Monoglobus and Desulfovibrio showed a positive significant (p < 0.05) correlation with total SCFA, indicating a high impact on gut fermentation. However, growth performance parameters and potential gas emission displayed no significant correlations with a specific bacterial genus. In conclusion, our results suggest that OC inclusion into pig diets could positively modulate and contribute to the gut microbiota’s favorable composition and functionality. Also, nutrient digestibility and gut fermentation patterns can be associated with specific microbial populations. |
| format | article |
| id | ReDivia8952 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers |
| publisherStr | Frontiers |
| record_format | dspace |
| spelling | ReDivia89522025-04-25T14:49:38Z Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions Belloumi, Dhekra García-Rebollar, Paloma Calvet, Salvador Francino, P. Reyes-Prieto, Mariana González-Garrido, Jorge Piquer, Laia Jiménez-Belenguer, Ana Isabel Bermejo, Almudena Cano, Carmen Cerisuelo, Alba Olive cake Fecal bacterial composition Gaseous emissions L02 Animal feeding Efficiency The microbial population in the pig’s gastrointestinal tract can be influenced by incorporating fibrous by-products into the diets. This study investigated the impact of including two types of dried olive cake (OC) in pigs’ diets on fecal bacterial composition. The correlation between fecal microbiota and growth performance, nutrient digestibility, gut fermentation pattern and slurry gas emissions was also evaluated. Thirty male Pietrain x (Landrace x Large white) pigs (47.9 ± 4.21 kg) were assigned to three groups: a control group (C), a group fed a diet with 20% partially defatted OC (20PDOC), and a group fed a diet with 20% cyclone OC (20COC) for 21 days. Fecal samples collected before and after providing the experimental diets were analyzed for the V3-V4 region of the 16S rRNA gene. Pigs were weighed, and feed intake was recorded throughout the study. Potential ammonia and methane emissions from slurry were measured. No significant differences in alpha diversity indexes were found. The taxonomic analysis revealed that Firmicutes and Bacteroidota phyla were dominant at the phylum level across all groups. Differential abundance analysis using ALDEx showed significant differences among groups for various bacteria at the phylum, genus, and species levels at the end of the experiment. Pigs from 20PDOC and 20COC groups exhibited increased abundances of health-promoting bacteria, such as Plactomycetota at the phylum level and Allisonella and an unidentified genus from the Eggerthellaceae family at the genus level. These changes influenced short-chain fatty acids’ (SCFA) concentration in slurries, leading to greater acetic, butyric, caproic and heptanoic acids in OC-fed groups, especially 20COC pigs. A volatility analysis revealed significant positive correlations (p < 0.05) between Uncultured_Bacteroidales and Unculured_Selenomonadaceae and energy digestibility. Monoglobus and Desulfovibrio showed a positive significant (p < 0.05) correlation with total SCFA, indicating a high impact on gut fermentation. However, growth performance parameters and potential gas emission displayed no significant correlations with a specific bacterial genus. In conclusion, our results suggest that OC inclusion into pig diets could positively modulate and contribute to the gut microbiota’s favorable composition and functionality. Also, nutrient digestibility and gut fermentation patterns can be associated with specific microbial populations. 2024-06-26T11:44:45Z 2024-06-26T11:44:45Z 2024 article publishedVersion Belloumi, D., García-Rebollar, P., Calvet, S., Francino, M. P., Reyes-Prieto, M., González-Garrido, J., ... & Cerisuelo, A. (2024). Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions. Frontiers in Microbiology, 15, 1359670. 1664-302X https://hdl.handle.net/20.500.11939/8952 10.3389/fmicb.2024.1359670 https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1359670/full en This work was funded by the Spanish Ministry of Science and Innovation (Project RTI-2018-095246-B-C22) and the project IVIA-GVA (52201L) from Instituto Valenciano de Investigaciones Agrarias (this project is co-financed by the European Union through the ERFF Program 2021–2027 Comunitat Valenciana). DB is a recipient of the Santiago Grisolía PhD scholarship (GRISOLIAP/2020/023) info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/E-RTA2018-095246/ES/UTILIZACION DE SUBPRODUCTOS FIBROSOS MEJORADOS EN PIENSOS DE PORCINO PARA INCREMENTAR LA SALUD INTESTINAL Y LA SOSTENIBILIDAD DE LA PRODUCCION PORCINA info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52201/ES/Relanzando la agroalimentación valenciana para una producción y consumo sostenibles y seguros/AgroAlimVal Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Frontiers electronico |
| spellingShingle | Olive cake Fecal bacterial composition Gaseous emissions L02 Animal feeding Efficiency Belloumi, Dhekra García-Rebollar, Paloma Calvet, Salvador Francino, P. Reyes-Prieto, Mariana González-Garrido, Jorge Piquer, Laia Jiménez-Belenguer, Ana Isabel Bermejo, Almudena Cano, Carmen Cerisuelo, Alba Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions |
| title | Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions |
| title_full | Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions |
| title_fullStr | Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions |
| title_full_unstemmed | Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions |
| title_short | Impact of including two types of destoned olive cakes in pigs’ diets on fecal bacterial composition and study of the relationship between fecal microbiota, feed efficiency, gut fermentation, and gaseous emissions |
| title_sort | impact of including two types of destoned olive cakes in pigs diets on fecal bacterial composition and study of the relationship between fecal microbiota feed efficiency gut fermentation and gaseous emissions |
| topic | Olive cake Fecal bacterial composition Gaseous emissions L02 Animal feeding Efficiency |
| url | https://hdl.handle.net/20.500.11939/8952 https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1359670/full |
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