Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.)
The involvement of effectors and transcriptional regulators in persimmon fruit maturation has been mostly approached by the literature under postharvest conditions. In order to elucidate the participation of these genes in the on-tree fruit maturation development, we have collected samples from seve...
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| Format: | article |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | https://hdl.handle.net/20.500.11939/8716 https://www.mdpi.com/2223-7747/12/16/2895 |
| _version_ | 1855032841292742656 |
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| author | Dorta, Tania Gil-Muñoz, Francisco Carrasco, Fany Zuriaga, Elena Ríos, Gabino Blasco, Manuel |
| author_browse | Blasco, Manuel Carrasco, Fany Dorta, Tania Gil-Muñoz, Francisco Ríos, Gabino Zuriaga, Elena |
| author_facet | Dorta, Tania Gil-Muñoz, Francisco Carrasco, Fany Zuriaga, Elena Ríos, Gabino Blasco, Manuel |
| author_sort | Dorta, Tania |
| collection | ReDivia |
| description | The involvement of effectors and transcriptional regulators in persimmon fruit maturation has been mostly approached by the literature under postharvest conditions. In order to elucidate the participation of these genes in the on-tree fruit maturation development, we have collected samples from seven persimmon germplasm accessions at different developmental stages until physiological maturation. This study has focused on the expression analysis of 13 genes involved in ethylene biosynthesis and response pathways, as well as the evolution of important agronomical traits such as skin colour, weight, and firmness. Results revealed different gene expression patterns, with genes up- and down-regulated during fruit development progression. A principal component analysis was performed to correlate gene expression with agronomical traits. The decreasing expression of the ethylene biosynthetic genes DkACO1, DkACO2, and DkACS2, in concordance with other sensing (DkERS1) and transduction genes (DkERF18), provides a molecular mechanism for the previously described high production of ethylene in immature detached fruits. On the other side, DkERF8 and DkERF16 are postulated to induce fruit softening and skin colour change during natural persimmon fruit ripening via DkXTH9 and DkPSY activation, respectively. This study provides valuable information for a better understanding of the ethylene signalling pathway and its regulation during on-tree fruit ripening in persimmon. |
| format | article |
| id | ReDivia8716 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia87162025-04-25T14:49:21Z Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.) Dorta, Tania Gil-Muñoz, Francisco Carrasco, Fany Zuriaga, Elena Ríos, Gabino Blasco, Manuel Transcriptional regulation Fruit ripening Softening F60 Plant physiology and biochemistry U10 Mathematical and statistical methods Ethylene Diospyros kaki The involvement of effectors and transcriptional regulators in persimmon fruit maturation has been mostly approached by the literature under postharvest conditions. In order to elucidate the participation of these genes in the on-tree fruit maturation development, we have collected samples from seven persimmon germplasm accessions at different developmental stages until physiological maturation. This study has focused on the expression analysis of 13 genes involved in ethylene biosynthesis and response pathways, as well as the evolution of important agronomical traits such as skin colour, weight, and firmness. Results revealed different gene expression patterns, with genes up- and down-regulated during fruit development progression. A principal component analysis was performed to correlate gene expression with agronomical traits. The decreasing expression of the ethylene biosynthetic genes DkACO1, DkACO2, and DkACS2, in concordance with other sensing (DkERS1) and transduction genes (DkERF18), provides a molecular mechanism for the previously described high production of ethylene in immature detached fruits. On the other side, DkERF8 and DkERF16 are postulated to induce fruit softening and skin colour change during natural persimmon fruit ripening via DkXTH9 and DkPSY activation, respectively. This study provides valuable information for a better understanding of the ethylene signalling pathway and its regulation during on-tree fruit ripening in persimmon. 2023-09-26T06:35:45Z 2023-09-26T06:35:45Z 2023 article publishedVersion Dorta, T., Gil-Muñoz, F., Carrasco, F., Zuriaga, E., Ríos, G. & Blasco, M. (2023). Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.). Plants, 12(16), 2895. 2223-7747 https://hdl.handle.net/20.500.11939/8716 10.3390/plants12162895 https://www.mdpi.com/2223-7747/12/16/2895 en This research was funded by Agencia Estatal de Investigación (AEI/10.13039/501100011033), grant number PID2020-113276RR-I00. T. D. was funded by the Spanish National Research Agency (MICIN/AEI/10.13039/501100011033) and the European Social Fund Plus (ESF+) (PRE2021-099546). M. B. was funded by PTQ2019-010663/AEI/10.13039/501100011033. info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-113276RR-100/ES/ESTUDIOS GENETICOS DE FRUTALES MENORES MEDITERRANEOS PARA MEJORA GENETICA DE SU ADAPTABILIDAD AL CAMBIO CLIMATICO info:eu-repo/grantAgreement/ESF/ESF+/PRE2021-099546 info:eu-repo/grantAgreement/AEI/Subprograma Estatal de Incorporación/PTQ2019-010663 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | Transcriptional regulation Fruit ripening Softening F60 Plant physiology and biochemistry U10 Mathematical and statistical methods Ethylene Diospyros kaki Dorta, Tania Gil-Muñoz, Francisco Carrasco, Fany Zuriaga, Elena Ríos, Gabino Blasco, Manuel Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.) |
| title | Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.) |
| title_full | Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.) |
| title_fullStr | Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.) |
| title_full_unstemmed | Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.) |
| title_short | Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Diospyros kaki L.) |
| title_sort | physiological changes and transcriptomic analysis throughout on tree fruit ripening process in persimmon diospyros kaki l |
| topic | Transcriptional regulation Fruit ripening Softening F60 Plant physiology and biochemistry U10 Mathematical and statistical methods Ethylene Diospyros kaki |
| url | https://hdl.handle.net/20.500.11939/8716 https://www.mdpi.com/2223-7747/12/16/2895 |
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