Development and Validation of a Duplex RT-qPCR for Detection of Peach Latent Mosaic Viroid and Comparison of Different Nucleic-Acid-Extraction Protocols

Peach latent mosaic viroid (PLMVd) is an important pathogen that causes disease in peaches. Control of this viroid remains problematic because most PLMVd variants are symptomless, and although there are many detection tests in use, the reliability of PCR-based methods is compromised by the complex,...

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Main Authors:	Luigi, Marta, Taglienti, Anna, Corrado, Carla Libia, Cardoni, Marco, Botti, Simona, Bissani, Rita, Casati, Paola, Passera, Alessandro, Miotti, Niccolò, De-Jonghe, Kris, Everaert, Ellen, Olmos, Antonio, Ruiz-García, Ana B., Faggioli, Francesco
Format:	Artículo
Language:	Inglés
Published:	MDPI 2023
Subjects:	Peach latent mosaic viroid Validated detection test TPS Rapid-extraction methods RT-PCR Low-cost diagnostic tests Nucleic-Acid-Extraction Protocols H20 Plant diseases U30 Research methods
Online Access:	https://hdl.handle.net/20.500.11939/8671 https://doi.org/10.3390/plants12091802