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Impact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices : implications for defining molecular markers of cell integrity

In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or from fermented dairy foods manufactured with these microorganisms. These experime...

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Autores principales: Pega, Juan Franco, Descalzo, Adriana Maria, Rossetti, Luciana, Nanni, Mariana, Perez, Carolina Daiana, Rizzo, Sergio Anibal, Diaz, Gabriela Perez
Formato: info:eu-repo/semantics/article
Lenguaje:Inglés
Publicado: 2017
Materias:
Acidos Nucléicos
Bacterias Acidolácticas
Marcadores Genéticos
Productos Lácteos
Nucleic Acids
Lactic Acid Bacteria
Genetic Markers
PCR
Milk Products
Marcadores Moleculares
Reacción en Cadena de la Polimerasa
Acceso en línea:http://hdl.handle.net/20.500.12123/1742
https://www.sciencedirect.com/science/article/pii/S002364381730155X
https://doi.org/10.1016/j.lwt.2017.03.010
Sumario:In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or from fermented dairy foods manufactured with these microorganisms. These experimental set-ups involved two starter lactic acid bacteria (SLAB), Streptococcus thermophilus and Lactococcus lactis, as models across their most common technological environments: yoghurt, milk and cheese. The presence of free DNA modified qPCR results in levels sufficient to bias SLAB determinations, indicating that DNA may be a poor measure of cellular integrity. Furthermore, we showed for the first time that an order of magnitude more of free RNA than of DNA had to be used to modify RT-qPCR results. Importantly, the interference produced by free DNA and RNA was maintained across all the dairy matrices evaluated, independently of the SLAB and of the source of nucleic acids used, in both negative and positive samples. This study suggests that, contrary to DNA molecules, mRNA from dead microorganisms or from other extracellular sources may not be a determinant factor in skewing molecular quantifications. Therefore, our results support the potential use of mRNA as a molecular signature of cell integrity in dairy matrices.

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